Study of the relationship between the biological activity of proteins and process conditions in bioproduct downstream operations

• Grant number: 12/23860-2
• Support Opportunities: Scholarships abroad - Research
• Start date: September 01, 2013
• End date: August 31, 2014
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Pedro de Alcântara Pessôa Filho
• Grantee: Pedro de Alcântara Pessôa Filho
• Host Investigator: John Prausnitz
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of California, Berkeley (UC Berkeley), United States

Abstract

This research proposal aims at the development of methodologies for the study of the biological activity of proteins in solution as a function of pH and temperature of this solution and of the ions existing thereon. The final objective is the design and/or improvement of models for protein downstream processing operations. The main issues to be addressed comprise the relationship between the distribution of protein molecule configurations and the activity curve as a function of pH (which entails the relationship between tertiary structure and activity), the development of equations to study the influence of the presence of ions, and the possible incorporation of terms related to the activation energy of the biological reactions and of the thermal denaturation onto the final equations. This project shall be conducted at the University of California, Berkeley, with the group of Prof. Dr. John Prausnitz. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PESSOA FILHO, PEDRO DE ALCANTARA; PRAUSNITZ, JOHN M.. A simplified electrostatic model for hydrolase catalysis. International Journal of Biological Macromolecules, v. 78, p. 257-265, JUL 2015. (12/23860-2)

MERCIER FRANCO, LUIS FERNANDO; PESSOA FILHO, PEDRO DE ALCANTARA. athematical Description of the Enzymatic Activity of Proteins with Ionizable Groups Exhibiting Deviations from the Henderson-Hasselbalch Equatio. Applied Biochemistry and Biotechnology, v. 194, n. 3, SI, OCT 2021. (12/23860-2)