Supramolecular assemblies of cationic lipid, antibiotics and polymers: preparation, characterization and activity against multiresistant bacteria and fast-growing mycobacteria

• Grant number: 12/24534-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: June 30, 2016
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Ana Maria Carmona-Ribeiro
• Grantee: Letícia Dias de Melo Carrasco
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The cationic lipid dioctadecyldimethylammonium bromide (DODAB) self-assembles as bilayer fragments (BF) or large bilayer vesicles (LV) in water depending on the dispersion method and are established as potent antimicrobial agents. On the other hand, vesicles and liposomes can be used as carriers for antibiotics able to deliver drugs inside cells such as the macrophages. Novel hybrid microbicidal materials are being developed by our group based on cationic lipid and polymers to address the problem of multiresistant bacteria and /or fast-growing mycobacteria. The novel supramolecular assemblies will be characterized by dynamic light scattering, determination of antibiotic incorporation from dialysis experiments, determination of bacteria viability by plating and CFU (colony forming unities) counting and determination of macrophages viability in the presence of bacteria and the novel formulations. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANCHES, LUCCAS MISSFELDT; SIQUEIRA PETRI, DENISE FREITAS; DE MELO CARRASCO, LETICIA DIAS; CARMONA-RIBEIRO, ANA MARIA. The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate). JOURNAL OF NANOBIOTECHNOLOGY, v. 13, SEP 24 2015. (12/24534-1, 11/00046-5)

CARMONA-RIBEIRO, ANA MARIA; DE MELO CARRASCO, LETICIA DIAS. Novel Formulations for Antimicrobial Peptides. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 15, n. 10, p. 18040-18083, OCT 2014. (12/24534-1)

DE MELO CARRASCO, LETICIA DIAS; MELLO SAMPAIO, JORGE LUIZ; CARMONA-RIBEIRO, ANA MARIA. Supramolecular Cationic Assemblies against Multidrug-Resistant Microorganisms: Activity and Mechanism of Action. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 16, n. 3, p. 6337-6352, 2015. (12/24534-1, 11/00046-5)

RAGIOTO, DANIELLE A. M. T.; CARRASCO, LETICIA D. M.; CARMONA-RIBEIRO, ANA M.. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 9, p. 3183-3192, 2014. (12/24534-1, 11/00046-5)

NAVES, ALLINY F.; PALOMBO, RENATA R.; CARRASCO, LETICIA D. M.; CARMONA-RIBEIRO, ANA M.. Antimicrobial Particles from Emulsion Polymerization of Methyl Methacrylate in the Presence of Quaternary Ammonium Surfactants. Langmuir, v. 29, n. 31, p. 9677-9684, AUG 6 2013. (12/24534-1, 11/00046-5)

RAGIOTO, DANIELLE A. M. T.; CARRASCO, LETICIA D. M.; CARMONA-RIBEIRO, ANA M.. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 9, p. 10-pg., 2014-01-01. (11/00046-5, 12/24534-1)

DE MELO CARRASCO, LETICIA DIAS; MELLO SAMPAIO, JORGE LUIZ; CARMONA-RIBEIRO, ANA MARIA. Supramolecular Cationic Assemblies against Multidrug-Resistant Microorganisms: Activity and Mechanism of Action. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 16, n. 3, p. 16-pg., 2015-03-01. (11/00046-5, 12/24534-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARRASCO, Letícia Dias de Melo. Supramolecular assemblies of cationic lipid, antibiotics and polymers: preparation, characterization and activity against multidrug resistant bacteria and fast growing mycobacteria.. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.