It is known that perennial plant species need to endure periodical changes in the environment, and in response they develop mechanisms allowing them to survive, through anatomical, molecular and cellular alterations. Stresses by extreme temperatures appear as one of the main elements that limit the geographical distribution and seasonal growth of many plants, affecting the quality and productivity of many crops and forest plantations. Cellulose and lignin are the primary polymers in plants. Eucalyptus is today the biggest source of cellulose in the world. Although lignin has a an important role in plants by providing support to cellulose microfibrils, it is a problem in the process to obtain cellulose. Brazil is the the largest producer of cellulose pulp and eucalyptus is the main source. This industry is responsible for generating more than 150,000 jobs in the country. The objective of this study is to evaluate the gene expression responses of E. urograndis and Eucalyptus globulus to low temperature (12oC) compared to control temperature of 25oC. The second species grow at lower temperatures and have impaired growth at higher temperatures. Its wood is of excellent quality, with high-yield pulp. Using a RNAseq database produced by our group, it will be investigated the differential expression of several genes related to the biosynthesis of lignin, through their identification and annotation, compared with sequences available in the literature, and validated their expression in experiments under controlled conditions.
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