Metagenomic analysis of periodontal subgingival microbiota in subjects with and without periodontal disease

• Grant number: 12/20915-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2013
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Magda Feres Figueiredo
• Grantee: Paula Juliana Perez Chaparro
• Host Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil

Abstract

Periodontitis is a polymicrobial infectious disease that has been reported as being a global health problem by the WHO. Research on the etiology of periodontitis has lasted for more than three centuries. Advances in microbiological diagnostic techniques have shown, with increasing resolution, the huge complexity of the interaction between the oral microbiota and the host. Molecular biology techniques have allowed for the detection of not yet cultured microorganisms in oral plaque samples, which has significantly increased our understanding of the etiology of periodontal disease. However, culture-independent techniques for cataloguing microbial species have mainly been based on PCR amplification of phylogenetic markers, e.g. 16S rDNA, which has generated intensive discussion in the literature concerning limitations related to the initial amplification steps. Next generation sequencing techniques have allowed for the direct sequencing of metagenomic DNA, reducing the biases incurred from gene cloning techniques. Until now, this approach has been used in only a few studies concerning the oral microbiota and only one has evaluated subjects without (n=3) or with (n=2) periodontal disease. These studies have yielded results that describe an oral microbial diversity orders of magnitude larger than from studies employing PCR and cloning. Thus, the present study aims to evaluate in detail the composition of subgingival plaque associated with periodontitis. This will be the first study to assess individually (non-pooled) plaque samples in subjects with and without periodontal disease using a metagenomic DNA direct sequencing approach.

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREZ-CHAPARRO, PAULA JULIANA; DUARTE, POLIANA MENDES; SHIBLI, JAMIL AWAD; MONTENEGRO, SHEYLA; HELUY, SILVIA LACERDA; FIGUEIREDO, LUCIENE CRISTINA; FAVERI, MARCELO; FERES, MAGDA. The Current Weight of Evidence of the Microbiologic Profile Associated With Peri-Implantitis: A Systematic Review. Journal of Periodontology, v. 87, n. 11, p. 1295-1304, NOV 2016. (12/20915-0)

PEREZ-CHAPARRO, P. J.; GONCALVES, C.; FIGUEIREDO, L. C.; FAVERI, M.; LOBAO, E.; TAMASHIRO, N.; DUARTE, P.; FERES, M.. Newly Identified Pathogens Associated with Periodontitis: A Systematic Review. JOURNAL OF DENTAL RESEARCH, v. 93, n. 9, p. 846-858, SEP 2014. (12/20915-0)

PEREZ-CHAPARRO, P. J.; DUARTE, P. M.; PANNUTI, C. M.; FIGUEIREDO, L. C.; MESTNIK, M. J.; GONCALVES, C. P. S.; FAVERI, M.; FERES, M.. Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette. Journal of Microbiological Methods, v. 111, p. 19-20, APR 2015. (12/20915-0)

GONCALVES, CRISTIANE; SOARES, GEISLA MARY S.; FAVERI, MARCELO; PEREZ-CHAPARRO, PAULA JULIANA; LOBAO, EDUARDO; FIGUEIREDO, LUCIENE CRISTINA; BACCELLI, GUSTAVO TITONELE; FERES, MAGDA. Association of three putative periodontal pathogens with chronic periodontitis in Brazilian subjects. Journal of Applied Oral Science, v. 24, n. 2, p. 181-185, MAR-APR 2016. (12/20915-0)

RATTO-TESPESTINI, ANNA CAROLINA; PEREZ-CHAPARRO, PAULA JULIANA; ROMITO, GUISEPPE ALEXANDRE; FIGUEIREDO, LUCIENE CRISTINA; FAVERI, MARCELO; CARILLO, HILANA PAULA; LARCHER, PRISCILA; FERES, MAGDA. Comparison of independent and dependent culture methods for the detection of transient bacteremia in diabetic subjects with chronic periodontitis. BIOMEDICA, v. 36, n. 1, p. 156-161, MAR 2016. (12/20915-0)

MCCULLOCH, JOHN ANTHONY; DE OLIVEIRA, VIVIANE MATOSO; DE ALMEIDA PINA, ANDRE VICIOLI; PEREZ-CHAPARRO, PAULA JULIANA; DE ALMEIDA, LARA MENDES; DE VASCONCELOS, JANAINA MOTA; DE OLIVEIRA, LAYANNA FREITAS; ANDRADE DA SILVA, DAISY ELAINE; GHISLAIN ROGEZ, HERVE LOUIS; CRETENET, MARINA; et al. Complete Genome Sequence of Lactococcus lactis Strain AI06, an Endophyte of the Amazonian Acai Palm. GENOME ANNOUNCEMENTS, v. 2, n. 6, p. 2-pg., 2014-11-01. (13/12107-4, 12/20915-0)

MCCULLOCH, JOHN ANTHONY; DE OLIVEIRA SILVEIRA, ALESSANDRO CONRADO; LIMA MORAES, ALINE DA COSTA; PEREZ-CHAPARRO, PAULA JULIANA; SILVA, MANOELLA FERREIRA; ALMEIDA, LARA MENDES; D'AZEVEDO, PEDRO ALVES; MAMIZUKA, ELSA MASAE. Complete Genome Sequence of Staphylococcus aureus FCFHV36, a Methicillin-Resistant Strain Heterogeneously Resistant to Vancomycin. GENOME ANNOUNCEMENTS, v. 3, n. 4, p. 2-pg., 2015-07-01. (13/12107-4, 12/20915-0)

DE ALMEIDA, LARA M.; PIRES, CARLOS; CERDEIRA, LOUISE T.; DE OLIVEIRA, THEO G. M.; MCCULLOCH, JOHN ANTHONY; PEREZ-CHAPARRO, PAULA JULIANA; SACRAMENTO, ANDREY G.; BRITO, ARTEMIR C.; DA SILVA, JOAS L.; DE ARAUJO, MARIA RITA E.; et al. Complete Genome Sequence of Linezolid-Susceptible Staphylococcus haemolyticus Sh29/312/L2, a Clonal Derivative of a Linezolid-Resistant Clinical Strain. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 3, MAY-JUN 2015. (12/16108-2, 13/06331-9, 13/12107-4, 12/20915-0)

MCCULLOCH, JOHN ANTHONY; DE OLIVEIRA SILVEIRA, ALESSANDRO CONRADO; LIMA MORAES, ALINE DA COSTA; PEREZ-CHAPARRO, PAULA JULIANA; SILVA, MANOELLA FERREIRA; ALMEIDA, LARA MENDES; D'AZEVEDO, PEDRO ALVES; MAMIZUKA, ELSA MASAE. Complete Genome Sequence of Staphylococcus aureus FCFHV36, a Methicillin-Resistant Strain Heterogeneously Resistant to Vancomycin. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 4, JUL-AUG 2015. (13/12107-4, 12/20915-0)

PEREZ-CHAPARRO, PAULA JULIANA; MCCULLOCH, JOHN ANTHONY; MAMIZUKA, ELSA MASAE; LIMA MORAES, ALINE DA COSTA; FAVERI, MARCELO; FIGUEIREDO, LUCIENE CRISTINA; DUARTE, POLIANA MENDES; FERES, MAGDA. Do different probing depths exhibit striking differences in microbial profiles?. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 45, n. 1, p. 26-37, JAN 2018. (13/12107-4, 12/20915-0, 13/23767-5)

ARETUZA FRITOLI; CRISTIANE GONÇALVES; MARCELO FAVERI; LUCIENE CRISTINA FIGUEIREDO; PAULA JULIANA PÉREZ-CHAPARRO; DAIANE FERMIANO; MAGDA FERES. The effect of systemic antibiotics administered during the active phase of non-surgical periodontal therapy or after the healing phase: a systematic review. Journal of Applied Oral Science, v. 23, n. 3, p. 249-254, 2015-06-00. (12/23731-8, 12/20915-0)

PEREZ-CHAPARRO, PAULA JULIANA; CERDEIRA, LOUISE TEIXEIRA; QUEIROZ, MAISE GOMES; SILVA DE LIMA, CLAYTON PEREIRA; LEVY, CARLOS EMILIO; PAVEZ, MONICA; LINCOPAN, NILTON; GONCALVES, EVONNILDO COSTA; MAMIZUKA, ELSA MASAE; MELLO SAMPAIO, JORGE LUIZ; et al. Complete Nucleotide Sequences of Two bla(KPC-2)-Bearing IncN Plasmids Isolated from Sequence Type 442 Klebsiella pneumoniae Clinical Strains Four Years Apart. Antimicrobial Agents and Chemotherapy, v. 58, n. 5, p. 2958-2960, MAY 2014. (13/12107-4, 12/20915-0)

DE ALMEIDA, LARA M.; PIRES, CARLOS; CERDEIRA, LOUISE T.; DE OLIVEIRA, THEO G. M.; MCCULLOCH, JOHN ANTHONY; PEREZ-CHAPARRO, PAULA JULIANA; SACRAMENTO, ANDREY G.; BRITO, ARTEMIR C.; DA SILVA, JOAS L.; DE ARAUJO, MARIA RITA E.; et al. Complete Genome Sequence of Linezolid-Susceptible Staphylococcus haemolyticus Sh29/312/L2, a Clonal Derivative of a Linezolid-Resistant Clinical Strain. GENOME ANNOUNCEMENTS, v. 3, n. 3, p. 1-pg., 2015-05-01. (12/16108-2, 13/12107-4, 12/20915-0, 13/06331-9)