Effect of whey proteins intake, peptides and amino acids in the heat shock proteins (HSPs) and related parameters in rats

• Grant number: 13/02862-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2013
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Jaime Amaya Farfan
• Grantee: Carolina Soares de Moura
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The whey protein (WP) has shown various properties and health effects. Recently, we reported that WP was able to increase expression of HSP70 in various tissues. The heat shock proteins (HSP) are composed of several families which represent a protective system that favors cellular resistance and tolerance before unfavorable conditions for the organism. It is believed that the high content of branched chain amino acids (BCAA) is an important factor involved in the ability of PSL increase in HSP70. The objective of this study is to investigate the influence of the consumption of whey protein as protein source in the diet on the expression of other HSPs, and also check the influence of amino acids and peptides present in the WP and associated parameters of HSPs. Methods: we will use in the first experiment, 48 male rats were divided primarily into 2 groups: sedentary and exercised. Each group is divided into three dietary groups (receiving AIN 93-G protein replacement): whey protein concentrate (WPC), whey protein hydrolyzate (WPH), casein (CAS) as a control. The animals of group exercised will be submitted to exercise on a treadmill as a source of heat stress. The following parameters will be determined: free amino acids in the plasma, HSP25, HSP60, HSP90, AHA1, DHFR, NOS, VEGF, PI3-Kinase, BCKDH, SOD, Catalase, GPx, p-AKT, GFAT, OGT, HSF1, HSF1 phosphorylated. In Experiment 2, we use 90 male rats were divided into 10 groups, which are gavados one of the following amino acids: leucine, valine, isoleucine, glutamine and arginine, or peptides: isoleucine-leucine, leucine-isoleucine, valine, leucine, leucine-valine in comparison with WPH. The animals will be subjected to the exercise as a source of stress. The following parameters are determined: free amino acids in the plasma, HSP25, HSP60, HSP90, HSP70, AHA1, DHFR, NOS, VEGF, PI3-Kinase, BCKDH, SOD, Catalase, GPx, p-AKT, GFAT, OGT, HSF1, HSF1 phosphorylated. The results will be statistically analyzed using ANOVA with significance set at p d 0.05, and Tukey's test. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; RISSO, EDER MULLER; AMAYA-FARFAN, JAIME. Modulatory effects of arginine, glutamine and branched-chain amino acids on heat shock proteins, immunity and antioxidant response in exercised rats. FOOD & FUNCTION, v. 8, n. 9, p. 3228-3238, SEP 2017. (16/06630-4, 13/02862-0, 13/06494-5)

MONTEIRO, NAICE E. S.; ROQUETTO, ALINE R.; DE PACE, FERNANDA; MOURA, CAROLINA S.; DOS SANTOS, ANDREY; YAMADA, AUREO T.; SAAD, MARIO JOSE A.; AMAYA-FARFAN, JAIME. Dietary whey proteins shield murine cecal microbiota from extensive disarray caused by a high-fat diet. Food Research International, v. 85, p. 121-130, JUL 2016. (13/02862-0, 12/05859-7)

MARINELI, RAFAELA DA SILVA; MOURA, CAROLINA SOARES; MORAES, ERICA AGUIAR; LENQUISTE, SABRINA ALVES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; AMAYA-FARFAN, JAIME; MAROSTICA, JR., MARIO ROBERTO. Chia (Salvia hispanica L.) enhances HSP, PGC-1 alpha expressions and improves glucose tolerance in diet-induced obese rats. NUTRITION, v. 31, n. 5, p. 740-748, MAY 2015. (13/02862-0, 12/23813-4, 11/13035-1)

MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; RISSO, EDER MULLER; AMAYA-FARFAN, JAIME. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. FOOD & NUTRITION RESEARCH, v. 61, 2017. (13/06494-5, 13/02862-0)

MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; RISSO, EDER MULLER; AMAYA-FARFAN, JAIME. Functional effects of milk bioactive peptides on skeletal muscle of rats. Food Research International, v. 84, p. 18-25, JUN 2016. (13/06494-5, 13/02862-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MOURA, Carolina Soares de. Effect of the intake of whey proteins, their peptides and amino acids on the heat shock proteins (HSPs) and health related parameters in rats. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.