Parkinson disease: possible involvement of kinin, purinergic and transient potential receptors.

• Grant number: 13/06044-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2013
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Principal Investigator: Luiz Roberto Giorgetti de Britto
• Grantee: Lívia Mendonça Munhoz Dati
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications, AP.TEM

Abstract

Parkinson's Disease (PD) is a neurodegenerative disease that involves loss of neurons in the substantia nigra, leading to effects such as bradykinesia, akinesia, resting tremor, muscular rigidity, gait and postural instability, and cognitive disabilities later. An animal model widely used to study the DP is the model that uses the direct administration of 6-OH-dopamine (6-OHDA) in the medial forebrain bundle or the striatum, this drug being responsible for the destruction of dopaminergic neurons selectively. There is evidence that some cellular communication systems can modulate the development of PD. For example, kinins, acting on B1 and B2 receptors may be involved in neuroprotection. Another type of receptor that may be involved in Parkinson purinergic receptors are activated by adenosine or ATP (P2 and P1 receptors). Also, receptors that have been associated with neurodegeneration channels are transient potential (TRPS) which are divided into subtypes such as TRPV, TRPM, TRPC, and TRPML TRPP. Taking into account the effects of these receptors on cells of the nervous system, this paper aims to evaluate the expression and involvement of these membrane receptors in PD induced by 6-OHDA in mice strain C57Bl/6 and knockout receptors B1, B2, TRPM2 and TRPM7. Moreover, agonists/antagonists of these receptors are also used to assess the issue, hoping that those molecules are involved in PD, playing possible protective action of the disease. For that techniques will be used as immunohistochemistry, Western blot and real-time PCR, which can generate relevant data toward the development of therapeutic strategies.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DATI, Lívia Mendonça Munhoz. Parkinson\'s disease: possible involvement of kinin, purine and transient potential receptors.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.