Antitumoral and toxicological potential of solamargine functionalized with yttrium vanadate nanoparticles

• Grant number: 13/02744-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2013
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Denise Crispim Tavares
• Grantee: Ricardo Andrade Furtado
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Chemotherapy is the use of chemicals for cancer treatment, however the side effects and drug resistance are well known problems in cancer therapy. These facts combined with cancer complexity, due to various genetic abnormalities, become an obstacle to the treatment of this disease. Thus, it is necessary the research and development of more effective drugs for cancer chemotherapy. The association with nanoparticles is an alternative to direct the drug to its action site, slowing the delivery of active molecules in the body and thus reduce systemic toxicity. Solamargine is the majority compound isolated from fruit of Solanum lycocarpum and has shown promising antitumor potential. In vitro studies have shown that solamargine was cytotoxic to cell lines of human colon carcinoma (HT29), human hepatoma (HepG2 and Hep3B) and breast cancer (HBL-100, ZR-75-1 and SK-BR-3). Additionally, a mixture of solasodines glycosylated, having the solamargine as the main component, reduced significantly non melanomic skin tumors in human when administered by intralesion injection. In order to increase the antitumor efficiency of solamargine and concomitantly reduce its cytotoxicity in normal cells, this study aims to evaluate the antitumor effect of solamargine functionalized with nanoparticles of yttrium vanadate on murine melanoma in C57BL/6 mice. Still, this study aims to evaluate the effects of this compound in different tissues for histopathologic, biochemistry and hematology analysis. To better understand the action mechanisms of functionalized solamargine in nanoparticles, will be also evaluated their genotoxicity potential by the comet assay in hepatocytes. This work will contribute to a better understanding of the action of solamargine, providing a more effective and safe use in future clinical applications. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FURTADO, RICARDO ANDRADE; OZELIN, SAULO DUARTE; FERREIRA, NATALIA HELEN; MIURA, BARBARA AYUMI; ALMEIDA JUNIOR, SILVIO; MAGALHAES, GEORGIA MODE; NASSAR, EDUARDO JOSE; MIRANDA, MARIZA ABREU; BASTOS, JAIRO KENUPP; TAVARES, DENISE CRISPIM. Antitumor activity of solamargine in mouse melanoma model: relevance to clinical safety. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 85, n. 4, OCT 2021. (13/02744-7, 13/13903-9, 19/02641-0)