Study of the role of ARHGAP21 protein in platelet formation and angiogenesis

• Grant number: 13/13022-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2013
• End date: May 19, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Sara Teresinha Olalla Saad
• Grantee: Vanessa Aline Bernusso
• Host Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The vascular system transmits and distributes hematopoietic cells, nutrients, gases and chemical mediators of angiogenesis, and participates in tumor development by providing oxygen and nutrients to cancer cells, promoting the formation of new vessels, which stimulate cancer growth and metastasis. Platelets are produced by hematopoietic cells in the bone marrow and play a role in angiogenesis through the storage transport and release of factors that regulate blood vessels growth. Another important function of the platelets is to initiate the repair of injured tissues by regulatory stimuli of platelet-platelet adhesion and platelet-endothelium. Microtubules, actin and protein Rho GTPase for RhoA are essential structural components in the biogenesis of platelets, participating in the formation of proplatelets. ARHGAP21 is a cell adhesion protein, which regulates actin, tubulin, controls the vesicular traffic in membranes of the Golgi complex and presents a possible role in tumor progression. Studies have reported that ARHGAP21 inhibition alters gene expression of endothelial cells and increases RhoA activity. Thus, the aim of this study is to analyze the role of ARHGAP21 protein in processes such as platelet formation and angiogenesis. Cell lines and primary cells from ARHAGAP21 knockout mice generated in our laboratory will be used.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERNUSSO, VANESSA ALINE; VIEIRA, KARLA P.; DUARTE, ADRIANA S. S.; LESCANO, CAROLINE HONAISER; MONICA, FABIOLA ZAKIA; VICENTE, CRISTINA PONTES; DE PAULA, ERICH VINICIUS; OLALLA SAAD, SARA TERESINHA; LAZARINI, MARIANA. eficiency of ARHGAP21 alters megakaryocytic cell lineage responses and enhances platelet hemostatic functio. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1868, n. 6, MAY 2021. (17/21801-2, 13/13022-2, 17/19674-2)

BERNUSSO, VANESSA ALINE; VIEIRA, KARLA P.; DUARTE, ADRIANA S. S.; LESCANO, CAROLINE HONAISER; MONICA, FABIOLA ZAKIA; VICENTE, CRISTINA PONTES; DE PAULA, ERICH VINICIUS; OLALLA SAAD, SARA TERESINHA; LAZARINI, MARIANA. Deficiency of ARHGAP21 alters megakaryocytic cell lineage responses and enhances platelet hemostatic function. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1868, n. 6, p. 13-pg., 2021-03-25. (17/19674-2, 13/13022-2, 17/21801-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERNUSSO, Vanessa Aline. Study of the role of ARHGAP21 during megakaryocytic differentiation and in the hemostatic process. 2021. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.