Terrestrial and aquatic plants for ecotoxicological evaluation of herbicides, insecticides and fungicides used in corn crop

• Grant number: 13/10462-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2013
• End date: September 30, 2014
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Claudinei da Cruz
• Grantee: Jaqueline Franciosi Della Vechia
• Host Institution: Centro Universitário da Fundação Educacional de Barretos (UNIFEB). Fundação Educacional de Barretos (FEBA). Barretos , SP, Brazil

Abstract

Environmental contamination caused by the increasing use and sometimes indiscriminate use of pesticides has generated concerns about the inappropriate release of these compounds into the environment. Bioindicator techniques have been used to demonstrate the presence and the possible environmental effects of these chemicals. The objective of this proposal is to evaluate the acute toxicity of three classes of pesticides tests using terrestrial plants, radish (Raphanus sativus), corn (Zea mays) and sunflower (Helianthus annuus ) and aquatic (Lemna minor and Azolla caroliniana) and effect on the emergence of the test plant lettuce (Lactuca sativa). The herbicides are atrazine, bentazone and clomazone, the insecticide will alfacipermetrine + teflubenzuron, and the tested will be a mixture of pyraclostrobin and pyraclostrobin + epoxiconazol. For the testing of phytotoxicity (LC50;14d) with terrestrial plants, will initially be sown in trays of seed germination of radish, corn and sunflower in organic substrate. After plant emergence, these will be kept in a greenhouse until the occurrence of the first pair of leaves. Phytotoxicity condition will be evaluated in bioassay and will be assessed at 1, 3, 5, 9 and 14 days after planting (DAP). Macrophyte Lemna minor and Azolla caroliniana be acclimated and after this period will also be conducted trials of acute toxicity (LC50;7d). Assessment of mortality percentage of the plants will be conducted on 3, 5 and 7 days after exposure to pesticides. For the germination test ten seeds of lettuce will be sown in washed sand containing increasing concentrations of pesticide evaluation of emergency will be assessed at 7, 14 and 21 days after sowing . With data from plant mortality and emergency will be calculated 50% lethal concentration of each pesticide. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DELLA VECHIA, J. F.; CRUZ, C.; SILVA, A. F.; CERVEIRA JR, W. R.; GARLICH, N.. Utilização de Bionsaios com Macrófitas no Biomonitoramento de Agrotóxicos em Ambiente Aquático. Planta Daninha, v. 34, n. 3, p. 597-603, JUL-SEP 2016. (13/10462-1)