The airway defense in young asymptomatic smokers

• Grant number: 13/11401-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2013
• End date: September 30, 2014
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Naomi Kondo Nakagawa
• Grantee: Carolina Tieko Yoshida
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Tobacco smoking inhalation is directly nocive to the respiratory epithelium, alters ciliary beating and the production and composition of mucus, promote ciliary morphological changes and loss of cilia, metaplasia and cheratinization of the goblet cells, followed by thickness of the epithelium and inflammation of the submucosa. Mucociliary transport (MCT) is an importante mechanism of pulmonar defense that removes the agentes from the airways and lungs. Mucus production increase and changes in physical properties may induce to mucus stasis in the respiratory tract that increases the área and time of contact between the respiratory epithelium and the microorganisms, that in turn predisposes to respiratory infections. The aim of the presente study is to assess the effects of smoking on the (1) MCT, (2) mucus physical properties and (3) exhaled breath condensate pH in young smokers as well as to analyze if these changes are associated with smoking history. Sixty subjects will be included (n=20 healthy non-smokers and 40 asymptomatic smokers) aged 18 to 35 y.o. that will be recruted at the Faculdade de Medicina da Universidade de São Paulo. Volunteers will be submitted to: (a) clinical examination and the questionnaire for upper airways symptoms (SNOT-20), (b) physical examination (arterial blood pressure, heart rate and pulse oximetry, (c) saccharine transit test, (d) nasal mucus collection for physical properties analyses, (e) nasal lavage collection for cotinine concentration analysis, (f) exhaled breath condensate collection for pH analysis and (g) lung function test. All variables will be analyzed by descriptive techniques. To compare data with normal distribution, we will use T Test. To compare nonparametric data between groups, we will use Mann-Whitney Test. For correlations between outcome variables and smoking history, we will use Pearson or Spearman coefficient. Significant differences will be considered if p< 0,05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICOLA, MARINA LAZZARI; DE CARVALHO, HERACLITO BARBOSA; YOSHIDA, CAROLINA TIEKO; DOS ANJOS, FABYANA MARIA; NAKAO, MAYUMI; SANTOS, UBIRATAN DE PAULA; MORAIS CARDOZO, KARINA HELENA; CARVALHO, VALDEMIR MELECHCO; PINTO, ERNANI; POLISELLI FARSKY, SANDRA HELENA; et al. Young ``Healthy{''} Smokers Have Functional and Inflammatory Changes in the Nasal and the Lower Airways. CHEST, v. 145, n. 5, p. 998-1005, MAY 2014. (13/13598-1, 13/11401-6)

NICOLA, MARINA LAZZARI; DE CARVALHO, HERACLITO BARBOSA; YOSHIDA, CAROLINA TIEKO; DOS ANJOS, FABYANA MARIA; NAKAO, MAYUMI; SANTOS, UBIRATAN DE PAULA; MORAIS CARDOZO, KARINA HELENA; CARVALHO, VALDEMIR MELECHCO; PINTO, ERNANI; POLISELLI FARSKY, SANDRA HELENA; et al. Young "Healthy" Smokers Have Functional and Inflammatory Changes in the Nasal and the Lower Airways. CHEST, v. 145, n. 5, p. 8-pg., 2014-05-01. (13/11401-6, 13/13598-1)