Experimental approaches in proteomics and glycomics applied to the characterization of Bothrops alcatraz

• Grant number: 13/14651-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2013
• End date: August 30, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Solange Maria de Toledo Serrano
• Grantee: Débora Andrade Silva
• Host Institution: Instituto Butantan. São Paulo , SP, Brazil
• Associated scholarship(s): 14/12245-0 - Glycomics applied to the characterization of Bothrops snake venoms, BE.EP.MS

Abstract

Snake venoms are important sources of active biomolecules whose scientific and therapeutic potential has not been fully explored. The primary biological function of the venom is to facilitate immobilization and digestion of prey. The observation of the existence of variability in the composition of snake venom proteomes at various taxonomic levels as well as the relationship between venom variability and animal diet remain in constant discussion. The Bothrops genus is widely distributed in the Brazilian territory, and the species B. jararaca is of great medical importance in the Southeastern region of the country. Genetic and phylogeographic analyses described the existence of a monophyletic group, named Jararaca group, which includes the island species B. alcatraz and B. insularis in addition to B. jararaca. Geological events during the Pleistocene (11,000 years ago) resulted in the geographic isolation of specimens of B. jararaca in different island environments in the Sao Paulo coast, leading to the emergence of B. alcatraz, on the Alcatrazes Island, and B. insularis, on the Queimada Grande Island. The restricted availability of food in these islands led to the existence of diets based on ectotherms, for juvenile specimens, and birds, for adult specimens of B. insularis, while B. alcatraz specimens are limited to ingestion of ectotherms, mainly centipedes and amphibians, throughout all life stages, so that even in adulthood, these individuals physically resemble the juvenile specimens of B. jararaca. Considering the absence of knowledge about the B. alcatraz venom, in this project we will use proteomics, peptidomics and glycomics approaches to explore the venom of B. alcatraz to analyze its composition in light of important analytical methodologies.

Scientific publications

(4)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

ANDRADE-SILVA, DEBORA; ASHLINE, DAVID; TRAN, THUY; LOPES, ALINE SORIANO; TRAVAGLIA CARDOSO, SILVIA REGINA; REIS, MARCELO DA SILVA; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.; REINHOLD, VERNON. Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes. MOLECULAR & CELLULAR PROTEOMICS, v. 17, n. 7, p. 1261-1284, JUL 2018. (13/07467-1, 14/12245-0, 13/14651-3, 13/13548-4)

ANDRADE-SILVA, DEBORA; NISHIYAMA JR, MILTON Y.; STUGINSKI, DANIEL R.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.. The distinct N-terminomes of Bothrops jararaca newborn and adult venoms. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1869, n. 7, JUL 2021. (16/16935-7, 13/07467-1, 13/14651-3)

ZELANIS, ANDRE; SILVA, DEBORA A.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.. A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 31, SEP 2019. (13/14651-3, 14/06579-3, 17/20106-9, 13/07467-1, 13/13548-4)

ANDRADE-SILVA, DEBORA; ZELANIS, ANDRE; TRAVAGLIA-CARDOSO, SILVIA R.; NISHIYAMA, JR., MILTON Y.; SERRANO, SOLANGE M. T.. Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates. JOURNAL OF PROTEOME RESEARCH, v. 20, n. 2, p. 1341-1358, FEB 5 2021. (13/13548-4, 16/16935-7, 13/07467-1, 13/14651-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Débora Andrade. Experimental approaches in proteomics and glycomics applied to the characterization of snake venom Bothrops alcatraz. 2016. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.