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Experimental approaches in proteomics and glycomics applied to the characterization of Bothrops alcatraz

Grant number: 13/14651-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): November 01, 2013
Effective date (End): August 30, 2015
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Solange Maria de Toledo Serrano
Grantee:Débora Andrade Silva
Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated scholarship(s):14/12245-0 - Glycomics applied to the characterization of Bothrops snake venoms, BE.EP.MS


Snake venoms are important sources of active biomolecules whose scientific and therapeutic potential has not been fully explored. The primary biological function of the venom is to facilitate immobilization and digestion of prey. The observation of the existence of variability in the composition of snake venom proteomes at various taxonomic levels as well as the relationship between venom variability and animal diet remain in constant discussion. The Bothrops genus is widely distributed in the Brazilian territory, and the species B. jararaca is of great medical importance in the Southeastern region of the country. Genetic and phylogeographic analyses described the existence of a monophyletic group, named Jararaca group, which includes the island species B. alcatraz and B. insularis in addition to B. jararaca. Geological events during the Pleistocene (11,000 years ago) resulted in the geographic isolation of specimens of B. jararaca in different island environments in the Sao Paulo coast, leading to the emergence of B. alcatraz, on the Alcatrazes Island, and B. insularis, on the Queimada Grande Island. The restricted availability of food in these islands led to the existence of diets based on ectotherms, for juvenile specimens, and birds, for adult specimens of B. insularis, while B. alcatraz specimens are limited to ingestion of ectotherms, mainly centipedes and amphibians, throughout all life stages, so that even in adulthood, these individuals physically resemble the juvenile specimens of B. jararaca. Considering the absence of knowledge about the B. alcatraz venom, in this project we will use proteomics, peptidomics and glycomics approaches to explore the venom of B. alcatraz to analyze its composition in light of important analytical methodologies.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZELANIS, ANDRE; SILVA, DEBORA A.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.. A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 31, . (13/14651-3, 14/06579-3, 17/20106-9, 13/07467-1, 17/07897-7, 13/13548-4)
ANDRADE-SILVA, DEBORA; ZELANIS, ANDRE; TRAVAGLIA-CARDOSO, SILVIA R.; NISHIYAMA, JR., MILTON Y.; SERRANO, SOLANGE M. T.. Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates. JOURNAL OF PROTEOME RESEARCH, v. 20, n. 2, p. 1341-1358, . (13/13548-4, 16/16935-7, 13/07467-1, 13/14651-3)
ANDRADE-SILVA, DEBORA; ASHLINE, DAVID; TRAN, THUY; LOPES, ALINE SORIANO; TRAVAGLIA CARDOSO, SILVIA REGINA; REIS, MARCELO DA SILVA; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.; REINHOLD, VERNON. Structures of N-Glycans of Bothrops Venoms Revealed as Molecular Signatures that Contribute to Venom Phenotype in Viperid Snakes. MOLECULAR & CELLULAR PROTEOMICS, v. 17, n. 7, p. 1261-1284, . (13/07467-1, 14/12245-0, 13/14651-3, 13/13548-4)
ANDRADE-SILVA, DEBORA; NISHIYAMA JR, MILTON Y.; STUGINSKI, DANIEL R.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.. The distinct N-terminomes of Bothrops jararaca newborn and adult venoms. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1869, n. 7, . (16/16935-7, 13/07467-1, 13/14651-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Débora Andrade. Experimental approaches in proteomics and glycomics applied to the characterization of snake venom Bothrops alcatraz. 2016. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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