PreditOmics - A Predictive Microbiology, Metagenomics and Metabolomics Approach in the Study of Microbiological Stability of Vacuum-Packaged Beef during Shelf-Life

• Grant number: 13/27118-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): April 01, 2014
• Effective date (End): July 31, 2014
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Anderson de Souza Sant'Ana
• Grantee: Humberto Moreira Hungaro
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In recent years, Brazil was consolidated as a leader in beef exports, with estimative that in 2020 it will supply 44.5 % of the world market. Despite promising growth prospects, there are still many obstacles in the production chain of Brazilian beef to be surpassed. Microbial spoilage is undoubtedly one of the major problems faced because of the intrinsic characteristics of meat and typical storage conditions. This process has been extensively studied, though most of the methods used are culture-dependent and do not allow complete identification of microbial species involved. "Omic" tools, such as metagenomics and metabolomics have the potential to revolutionize the studies of microbial ecology in order to allow a greater understanding of the spoilage process. Despite this, there is still a lack of studies on the microbial ecology of Brazilian beef vacuum packed, especially in dark cutters. The incidence of dark firm dry (DFD) meat is high in Brazil and their intrinsic characteristics favor the growth of microbial groups, leading to a reduced shelf life. Thus, this project will investigate the diversity and microbial succession of Brazilian vacuum-packaged beef during storage, through an approach combining metagenomics, metabolomics and predictive modeling. The outputs of the project are expected to contribute to the understanding of microbial ecology of Brazilian vacuum-packaged beef as well as to generate scientific information to implement measures aiming at establishing quality standards for this product.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FURTADO, MARIANNA M.; SILVA, BEATRIZ S.; FREIRE, LUISA; GRACA, JULIANA S.; ALVARENGA, VERONICA O.; HUNGARO, HUMBERTO M.; SANT'ANA, ANDERSON S.. Investigating desiccation resistance, post-rehydration growth, and heat tolerance in desiccation-injured cells of Salmonella enterica isolated from the soybean production chain. International Journal of Food Microbiology, v. 405, p. 8-pg., 2023-11-16. (13/27118-1, 16/21041-5, 16/12001-0)

HUNGARO, HUMBERTO M.; CATURLA, MAGDEVIS Y. R.; HORITA, CLAUDIA N.; FURTADO, MARIANNA M.; SANT'ANA, ANDERSON S.. Blown pack spoilage in vacuum-packaged meat: A review on clostridia as causative agents, sources, detection methods, contributing factors and mitigation strategies. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 52, p. 123-138, JUN 2016. (13/27118-1, 14/25028-8)

CHAVES, RAFAEL D.; KUMAZAWA, SAMUEL H.; KHANEGHAH, AMIN MOUSAVI; ALVARENGA, VERONICA O.; HUNGARO, HUMBERTO M.; SANT'ANA, ANDERSON S.. Comparing the susceptibility to sanitizers, biofilm-forming ability, and biofilm resistance to quaternary ammonium and chlorine dioxide of 43 Salmonella enterica and Listeria monocytogenes strains. FOOD MICROBIOLOGY, v. 117, p. 8-pg., 2023-09-08. (13/27118-1, 18/15432-7, 14/03791-1, 17/00569-4)