Stochastic Single Cell Modeling of Biofilm Formation and Inactivation by Sanitizers of Salmonella and Listeria monocytogenes and their Impacts on Food Safety

• Grant number: 14/03791-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2014
• End date: April 30, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
• Principal Investigator: Anderson de Souza Sant'Ana
• Grantee: Rafael Djalma Chaves
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/20821-7 - Stochastic modelling of Salmonella and Listeria monocytogenes resistance to sanitizers: single cell and molecular approaches, BE.EP.PD

Abstract

Studies have shown that cross contamination/recontamination during food processing is one of the main factors contributing to the occurrence of foodborne disease outbreaks (FBDs). Cross contamination/recontamination is associated with persistence of Salmonella and Listeria monocytogenes in food processing environments, wich is remarkable linked to the ability of these microorganisms to form biofilms. The persistence phenomenon can also be attributed to an increased tolerance to sanitizers or due to a greater adhesion ability to abiotic surfaces by a given clone (strain) of a pathogen. Despite the advances in understanding the impact of variability between strains, for example, in the ability to form biofilms, it is known that this approach has several limitations, of which the fact that the responses are considered for the microbial population as a whole and that differences among individual cells are not taken into account. The importance of understanding the behaviour and variability between individual cells precisely emerges with the emergence of quantitative risk assessment. It is understood that approaches to understand the variability among individual cells with respect to their ability to form biofilms and to resist to sanitizing agents are of utmost importance for improving the efficiency of cleaning procedures. Thus, this project aims to: i) to use stochastic single cell modeling to study the resistance of Salmonella and L. monocytogenes to different sanitizers ii) to use stochastic single cell modeling to assess the resistance to sanitizes of sub-populations of one specific strain of each pathogen and iii) to use stochastic single cell modeling to study biofilm formation by Salmonella and L. monocytogenes.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, MONYCA D.; CHAVES, RAFAEL D.; FREIRE, LUISA; PIA, ARTHUR K. R.; FURTADO, MARIANNA M.; ALVARENGA, VERONICA O.; CRUCELLO, ALINE; LOPES, LETICIA S.; SANTOS, ANDRE F. M.; RODRIGUES, DALIA P.; et al. Salmonella enterica in soybean production chain: Occurrence, characterization, and survival during soybean storage. International Journal of Food Microbiology, v. 372, p. 12-pg., 2022-07-02. (14/03791-1, 16/21041-5)

CHAVES, RAFAEL D.; ASPRIDOU, ZAFEIRO; SANT'ANA, ANDERSON S.; KOUTSOUMANIS, KONSTANTINOS P.. Effect of chlorine stress on the subsequent growth behavior of individual Salmonella cells. Food Research International, v. 123, p. 311-316, SEP 2019. (16/20821-7, 14/03791-1)

SANTOS, JULIANA L. P.; CHAVES, RAFAEL D.; SANT'ANA, ANDERSON S.. Estimation of growth parameters of six different fungal species for selection of strains to be used in challenge tests of bakery products. FOOD BIOSCIENCE, v. 20, p. 62-66, DEC 2017. (14/03791-1)

BATISTA, MARCOS R. D.; UNO, FABIANA; CHAVES, RAFAEL D.; TIDON, ROSANA; ROSA, CARLOS A.; KLACZKO, LOUIS B.. Differential attraction of drosophilids to banana baits inoculated with Saccharomyces cerevisiae and Hanseniaspora uvarum within a Neotropical forest remnant. PeerJ, v. 5, MAR 9 2017. (12/03144-0, 14/03791-1)

MUNFORD, ALLAN R. G.; ALVARENGA, VERONICA O.; DO PRADO-SILVA, LEONARDO; CRUCELLO, ALINE; CAMPAGNOLLO, FERNANDA B.; CHAVES, RAFAEL D.; OTEIZA, JUAN M.; SANT'ANA, ANDERSON S.. Sporeforming bacteria in beer: Occurrence, diversity, presence of hop resistance genes and fate in alcohol-free and lager beers. FOOD CONTROL, v. 81, p. 126-136, NOV 2017. (14/17387-8, 14/14891-7, 14/03791-1)

SANTOS, JULIANA L. P.; CHAVES, RAFAEL D.; SANT'ANA, ANDERSON S.. Modeling the impact of water activity, pH, and calcium propionate on the germination of single spores of Penicillium paneum. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 133, NOV 2020. (14/03791-1)

MORASSI, LETICIA L. P.; BERNARDI, ANGELICA O.; AMARAL, ALEXANDRA L. P. M.; CHAVES, RAFAEL D.; SANTOS, JULIANA L. P.; COPETTI, MARINA V.; SANT'ANA, ANDERSON S.. Fungi in cake production chain: Occurrence and evaluation of growth potential in different cake formulations during storage. Food Research International, v. 106, p. 141-148, APR 2018. (14/03791-1)

MORASSI, L. P. LETICIA; SILVA, S. BEATRIZ; FURTADO, M. MARIANNA; FREIRE, LUISA; SANTOS, L. P. JULIANA; CHAVES, D. RAFAEL; GRANATO, DANIEL; SILVA, P. MIRIAM; PEN, E. L. WILMER; ANA, ANDERSON S. SANT'. Growth/no-growth modeling to control the spoilage of chocolate cake by Penicillium citrinum LMQA_053: Impact of pH, water activity, temperature, and different concentrations of calcium propionate and potassium sorbate. FOOD CONTROL, v. 139, p. 7-pg., 2022-09-01. (14/03791-1, 16/21041-5)

CHAVES, RAFAEL D.; KUMAZAWA, SAMUEL H.; KHANEGHAH, AMIN MOUSAVI; ALVARENGA, VERONICA O.; HUNGARO, HUMBERTO M.; SANT'ANA, ANDERSON S.. Comparing the susceptibility to sanitizers, biofilm-forming ability, and biofilm resistance to quaternary ammonium and chlorine dioxide of 43 Salmonella enterica and Listeria monocytogenes strains. FOOD MICROBIOLOGY, v. 117, p. 8-pg., 2023-09-08. (13/27118-1, 18/15432-7, 14/03791-1, 17/00569-4)