Identification of microRNAs involved in meat tenderness in Nellore cattle

• Grant number: 14/00943-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2014
• End date: July 31, 2015
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Luiz Lehmann Coutinho
• Grantee: Berna Ines Gimenez Kappeler
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 12/23638-8 - Molecular basis of meat quality in Nelore beef cattle, AP.TEM

Abstract

Brazil is the world's second largest producer of beef, just after the United States, and exports increase is related to the implementation of new tools for the selection of zebu animals (Bos taurus indicus) that accounts for 80% of Brazil's meat exports. Meat tenderness is one of the most important organoleptic characteristic for consumers, and more knowledge of the biological processes involved in this phenotype is required. The advent of next-generation sequencing technology has provided tools to study the genome on a large scale, more quickly and less costly. This project aims to identify changes in expression of microRNAs associated with meat tenderness in Nellore cattle. Animals from a population generated in a previous research project of the Network BifeQuali Embrapa, completed in 2012, will be used. Samples of muscle tissue were collected and the phenotypes were measured. The total RNA from 34 animals with extreme shear force values were extracted and microRNA libraries of these samples will be sequenced on illumina MiSeq equipment. Finally, the differential expression analysis will be performed using bioinformatics tools.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KAPPELER, BERNA I. G.; REGITANO, LUCIANA C. A.; POLETI, MIRELE D.; CESAR, ALINE S. M.; MOREIRA, GABRIEL C. M.; GASPARIN, GUSTAVO; COUTINHO, LUIZ L.. MiRNAs differentially expressed in skeletal muscle of animals with divergent estimated breeding values for beef tenderness. BMC MOLECULAR BIOLOGY, v. 20, JAN 3 2019. (12/23638-8, 13/21017-9, 14/00943-5, 14/11871-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

KAPPELER, Berna Ines Gimenez. Identification of microRNAs involved in meat tenderness in Nellore cattle. 2015. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.