In vivo and in vitro evaluation of chemo preventive potential of Copaifera reticulata Ducke oil-resin: genotoxic, biochemical and immunohistochemical parameters

• Grant number: 13/19488-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2014
• End date: April 30, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal Investigator: Jairo Kenupp Bastos
• Grantee: Juliana Marques Senedese Dias
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM

Abstract

Interest in the medicinal plant research has grown in recent years, primarily for the treatment of diseases that have a high social impact , such as cancer . Since long been recognized that inflammation is a risk factor for certain cancers . Inflammation by itself does not cause cancer , mutations and epigenetic changes , arising from environmental exposure or immune changes , contribute to the carcinogenic process . Several pro-inflammatory mediators such as prostaglandins , nitric oxide (NO) signaling cascades disrupt the cells , which contributes to the development of neoplasms . Along with these mediators, the nuclear transcription factor kappa B ( NF - kB ) are persistently active in diseases such as cancer , arthritis , chronic inflammation , and outras.As Copaifera species of the genus are known popularly as " copal " , " capaiva " or " stick-to - oil " , and are found in fields and pastures in northern and northeastern Brazil . The oil - copal resin is widely known in Brazil for its therapeutic activity based on popular use in highlighting the healing activity and anti - inflammatory . Among the various species of the genus , the literature reports the anti-inflammatory activity of oleoresin of C. reticulata Ducke Thus , correlating the fact that the inflammatory process being envolvidono cancer development , this paper proposes to evaluate the chemopreventive potential of oleoresin of C. reticulata Ducke in rats , as well as identification of molecular pathways modulated by this natural product . Therefore , it will be analyzed the genotoxic effect through the comet assay in colon cells . The chemopreventive potential of oleoresin on colon carcinogenesis induced by the carcinogen 1,2- dimethylhydrazine will be assessed using the comet assay and testing of aberrant crypt foci . Still be studied immunostaining of cyclooxygenase -2 and NF -kB using immunohistochemical analysis in colon. The anti-inflammatory activity also will be assessed the action of oleoresin beyond their major compounds , through the quantification of NO and prostaglandin E2 in cultured RAW 264.7 macrophage lineage , NP . Achieving this project will contribute to a better understanding of biological activities and mechanisms of action of the oil - resin Copaifera , which is of great importance ethnopharmacological

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SENEDESE, JULIANA MARQUES; RINALDI-NETO, FRANCISCO; FURTADO, RICARDO ANDRADE; NICOLLELA, HELOIZA DINIZ; RIBEIRO DE SOUZA, LARISSA DANIELA; RIBEIRO, ARTHUR BARCELOS; FERREIRA, LUCAS SOUZA; MAGALHAES, GEORGIA MODE; CARLOS, IRACILDA ZEPPONE; MANGABEIRA DA SILVA, JONAS JOAQUIM; et al. Chemopreventive role of Copaifera reticulata Ducke oleoresin in colon carcinogenesis. BIOMEDICINE & PHARMACOTHERAPY, v. 111, p. 331-337, MAR 2019. (11/13630-7, 13/19488-3)