| Grant number: | 13/19488-3 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | May 01, 2014 |
| Effective date (End): | April 30, 2017 |
| Field of knowledge: | Health Sciences - Pharmacy - Pharmacognosy |
| Principal Investigator: | Jairo Kenupp Bastos |
| Grantee: | Juliana Marques Senedese Dias |
| Home Institution: | Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
| Associated research grant: | 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM |
Abstract Interest in the medicinal plant research has grown in recent years, primarily for the treatment of diseases that have a high social impact , such as cancer . Since long been recognized that inflammation is a risk factor for certain cancers . Inflammation by itself does not cause cancer , mutations and epigenetic changes , arising from environmental exposure or immune changes , contribute to the carcinogenic process . Several pro-inflammatory mediators such as prostaglandins , nitric oxide (NO) signaling cascades disrupt the cells , which contributes to the development of neoplasms . Along with these mediators, the nuclear transcription factor kappa B ( NF - kB ) are persistently active in diseases such as cancer , arthritis , chronic inflammation , and outras.As Copaifera species of the genus are known popularly as " copal " , " capaiva " or " stick-to - oil " , and are found in fields and pastures in northern and northeastern Brazil . The oil - copal resin is widely known in Brazil for its therapeutic activity based on popular use in highlighting the healing activity and anti - inflammatory . Among the various species of the genus , the literature reports the anti-inflammatory activity of oleoresin of C. reticulata Ducke Thus , correlating the fact that the inflammatory process being envolvidono cancer development , this paper proposes to evaluate the chemopreventive potential of oleoresin of C. reticulata Ducke in rats , as well as identification of molecular pathways modulated by this natural product . Therefore , it will be analyzed the genotoxic effect through the comet assay in colon cells . The chemopreventive potential of oleoresin on colon carcinogenesis induced by the carcinogen 1,2- dimethylhydrazine will be assessed using the comet assay and testing of aberrant crypt foci . Still be studied immunostaining of cyclooxygenase -2 and NF -kB using immunohistochemical analysis in colon. The anti-inflammatory activity also will be assessed the action of oleoresin beyond their major compounds , through the quantification of NO and prostaglandin E2 in cultured RAW 264.7 macrophage lineage , NP . Achieving this project will contribute to a better understanding of biological activities and mechanisms of action of the oil - resin Copaifera , which is of great importance ethnopharmacological | |