Effect of hypoxia during late phase of incubation on post-hatching lung structures in broiler

• Grant number: 14/01861-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2014
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Kênia Cardoso Bícego
• Grantee: Lara do Amaral Silva
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

There is evidence of the effects of hypoxia during different phases of incubation on the development of organs and energetic metabolism of chicken hatchlings. However, no study investigated the effect of hypoxia during late phase of incubation in the lung morphology of chicks. Thus, we will use stereology technique to verify the effect of hypoxia (15%O2) on lung structure of hatchlings and 10 days old chicks. To this end, fertile eggs (Cobb 500 strain) will be incubated in normoxia during entire incubation (Nx) or in hypoxia in the last week of incubation.(HxL). Hatchlings and 10 days old chicks will be euthanized, lungs fixed and lung volume determined using Cavalieri technique. Cubes of 2/2mm in resin will be cut for light and transmission electron microscopy (sequential cuts in crescent angles). Following images it will be possible to determine volume of structures and quantify barrier and surface for gas exchanges.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO AMARAL-SILVA, LARA; LAMBERTZ, MARKUS; ZARA, FERNANDO JOSE; KLEIN, WILFRIED; GARGAGLIONI, LUCIANE HELENA; BICEGO, KENIA CARDOSO. Parabronchial remodeling in chicks in response to embryonic hypoxia. Journal of Experimental Biology, v. 222, n. 10, MAY 2019. (13/13386-4, 14/01861-2, 08/57712-4)

DO AMARAL-SILVA, LARA; SCARPELLINI, CAROLINA DA S.; TORO-VELASQUEZ, PAULA ANDREA; FERNANDES, MARCIA H. M. R.; GARGAGLIONI, LUCIANE H.; BICEGO, KENIA C.. Hypoxia during embryonic development increases energy metabolism in normoxic juvenile chicks. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 207, p. 93-99, MAY 2017. (11/19131-2, 10/20285-1, 14/01861-2, 11/07509-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Lara do Amaral. Structural remodeling of parabronchial tissue of chicks in response to hypoxia during fetal development. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.