Surface treatment of cpTi with plasma electrolytic oxidation and glow discharge plasma: characterization and multispecies biofilm development

• Grant number: 13/26145-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2014
• End date: February 29, 2016
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Valentim Adelino Ricardo Barão
• Grantee: Adaias Oliveira Matos
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The aims of this study are to investigate the physical-chemical properties and multispecies biofilm development on commercially pure titanium (cpTi) with different surface treatments: I - machined (control); II - modified by aluminum oxide (Al2O3) sandblasting followed by chloride acid (HCl) and hydrogen peroxide treatment (H2O2) (control - commercially available dental implant surface); III - modified by plasma electrolytic oxidation with calcium and phosphate solution (experimental surface) and IV - modified by glow discharge plasma using oxygen (O2) and hexamethyldisiloxane (HMDSO) (experimental surface). Discs' surfaces will be characterized by Scanning Electron Microscopy (SEM), white light interferometry microscopy, and their surface free energy and surface roughness will be investigated. Energy Dispersive Spectroscopy (EDS) and X-Ray Diffraction analysis (XRD) will be used to investigate the composition of the oxide layer formed on the cpTi surface. In the microbiological assay, multispecies biofilm of Streptococcus sanguinis, Actinomyces naeslundii and Fusobacterium nucleatum will grow on discs' surfaces during 2 hours (adhesion) and 48 hours (biofilm). The colony forming units will be determined by specific mediums (log CFU/cm2). The biofilm biomass will be measured with the crystal violet chromogenic assay, and the composition of the extracellular matrix (proteins and carbohydrates) will be investigated by the biocinchoninic acidic and phenol-sulfuric assays. SEM images of the biofilm formed on the cpTi discs will be used to investigate the distribution of microorganisms onto each surface. Quantitative data will be statistically analyzed at a significant level of 5%. The number of samples for each assay will be determined by a pilot study. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATOS, ADAIAS O.; RICOMINI-FILHO, ANTONIO P.; BELINE, THAMARA; OGAWA, ERIKA S.; COSTA-OLIVEIRA, BARBARA E.; DE ALMEIDA, AMANDA B.; NOCITI JUNIOR, FRANCISCO H.; RANGEL, ELIDIANE C.; DA CRUZ, NILSON C.; SUKOTJO, CORTINO; et al. Three-species biofilm model onto plasma-treated titanium implant surface. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 152, p. 354-366, APR 1 2017. (13/08451-1, 13/26145-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MATOS, Adaias Oliveira. Surface treatment of cpTi with micro-arc oxidation and glow discharge plasma: characterization and multispecies biofilm development. 2016. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.