Development of a bioactive and antibacterial surface containing Cu2O for dental implants

• Grant number: 17/01320-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): May 01, 2017
• Effective date (End): April 30, 2021
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Valentim Adelino Ricardo Barão
• Grantee: Jairo Matozinho Cordeiro
• Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated scholarship(s): 19/07956-9 - Biological response of biomaterials loaded with copper ions for dental implants rehabilitation, BE.EP.DR

Abstract

The search for dental implants surfaces that prevent infections and improve the biological properties is still a challenging. This study aims to develop bioactive and antibacterial surfaces containing cooper oxide (Cu2O) onto commercially pure titanium (cpTi) discs by means of plasma electrolytic oxidation (PEO). CpTi discs (15 mm diameter and 2 mm thickness) will be subjected to different surface modifications: machined (surface I - control); modified by PEO with calcium and phosphate solution (surface II); modified by PEO with solution of calcium, phosphate and Cu2O nanoparticles (surface III). The surfaces will be characterized as surface roughness (Ra, Rq, Rt, Rz), atomic force microscopy, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction analysis (XRD), X-ray photoelectron spectroscopy, wettability and surface free energy. For the electrochemical assay, standard tests (open circuit potential, electrochemical impedance spectroscopy, and potentiodynamic tests) will be conducted in body fluid solution (pH 7,4) to understand the corrosion kinetics and to investigate the properties of the oxide film formed on the discs surface. For biological tests, murine osteoprogenitor cell line (MC3T3E1) will be used. The cell structure and morphology will be evaluated by SEM. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) will be used to check the levels of osteogenic genes expression. MTT assay will be used to assess cell viability. The influence of surface treatment on the adsorption of serum proteins (albumin, fibrinogen and fibronectin) will be determined by the bicinchoninic acidic method. For microbiological test, the biofilm composed of Streptococcus sanguinis will be formed onto the discs surfaces. The log of colony forming units will be determined (log CFU), and the biofilm structure will be analyzed by SEM. Quantitative data will be submitted to the most appropriate statistical analysis with 5% significance level. The number of specimens for each test will be determined after the pilot study. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORDEIRO, JAIRO M.; PIRES, JULIA M.; SOUZA, JOAO G. S.; LIMA, CAROLINA V.; BERTOLINI, MARTINNA M.; RANGEL, ELIDIANE C.; BARAO, VALENTIM A. R. Optimizing citric acid protocol to control implant-related infections: An in vitro and in situ study. JOURNAL OF PERIODONTAL RESEARCH, v. 56, n. 3 FEB 2021. Web of Science Citations: 0.

DINI, CAROLINE; NAGAY, BRUNA E.; CORDEIRO, JAIRO M.; DA CRUZ, NILSON C.; RANGEL, ELIDIANE C.; RICOMINI-FILHO, ANTONIO P.; DE AVILA, ERICA D.; BARAO, VALENTIM A. R. UV-photofunctionalization of a biomimetic coating for dental implants application. Materials Science & Engineering C-Materials for Biological Applications, v. 110, MAY 2020. Web of Science Citations: 0.

NAGAY, BRUNA E.; DINI, CAROLINE; CORDEIRO, JAIRO M.; RICOMINI-FILHO, ANTONIO P.; DE AVILA, ERICA D.; RANGEL, ELIDIANE C.; DA CRUZ, NILSON C.; BARAO, VALENTIM A. R. Visible-Light-Induced Photocatalytic and Antibacterial Activity of TiO2 Codoped with Nitrogen and Bismuth: New Perspectives to Control Implant-Biofilm-Related Diseases. ACS APPLIED MATERIALS & INTERFACES, v. 11, n. 20, p. 18186-18202, MAY 22 2019. Web of Science Citations: 0.

CORDEIRO, JAIRO M.; NAGAY, BRUNA E.; RIBEIRO, ANA LUCIA R.; DA CRUZ, NILSON C.; RANGEL, ELIDIANE C.; FAIS, LAIZA M. G.; VAZ, LUIS G.; BARAO, VALENTIM A. R. Functionalization of an experimental Ti-Nb-Zr-Ta alloy with a biomimetic coating produced by plasma electrolytic oxidation. Journal of Alloys and Compounds, v. 770, p. 1038-1048, JAN 5 2019. Web of Science Citations: 7.

CORDEIRO, JAIRO M.; PANTAROTO, HELOISA N.; PASCHOALETO, EMANUELLA M.; RANGEL, ELIDIANE C.; DA CRUZ, NILSON C.; SUKOTJO, CORTINO; BARAO, VALENTIM A. R. Synthesis of biofunctional coating for a TiZr alloy: Surface, electrochemical, and biological characterizations. Applied Surface Science, v. 452, p. 268-278, SEP 15 2018. Web of Science Citations: 4.