Radiomics and transcriptomics analysis of breast cancer in an animal model after melatonin treatment

• Grant number: 14/13815-5
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): January 01, 2015
• Effective date (End): November 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Debora Aparecida Pires de Campos Zuccari
• Grantee: Bruna Victorasso Jardim-Perassi
• Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 15/18541-3 - Radiomic analysis of breast cancer animal model after melatonin treatment, BE.EP.PD

Abstract

Breast cancer is the most common cancer in women being rated as the second most frequent type of cancer in the world. Melatonin is a hormone naturally produced by the pineal gland and currently has been related to cancer. Studies suggest its therapeutic use in different types of cancer, but still need a better understanding of their mechanisms of action and oncostatic effects. In this context, this proposal is part of a Thematic Project submitted to FAPESP,and aims to assess the action of melatonin by transcriptomics and radiomics analysis with emphasis on intratumor heterogeneity for understanding the effectiveness of melatonin treatment in an experimental model of breast cancer. Athymic nude mice will receivethe implantation of breast cancer cells MDA-MB-231 and will be treated with melatonin or vehicle. At the end of treatment, the analysis of the transcriptome will be held in tumor tissue by RNA-Seq (Next-generation sequencing technology). The most pronounced changes will be validated individually, especially preferably those involved in pathways of angiogenesis and changes in the cellular energy metabolism (hypoxia and acidosis), as these are directly related to the characterization of intratumor heterogeneity, the main objective of this study. Also, will be used the radiomics, with quantitative analysis of images and subsequent comparison with biological characteristics of the tumor, to verify the intratumor heterogeneity and the influence of melatonin in this process. The results may help to elucidate numerous mechanisms of action of melatonin, strengthening its use as a therapeutic agent in breast cancer. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JARDIM-PERASSI, BRUNA VICTORASSO; ALEXANDRE, PAMELA A.; SONEHARA, NATHALIA M.; DE PAULA-JUNIOR, RUBENS; REIS JUNIOR, OSVALDO; FUKUMASU, HEIDGE; CHAMMAS, ROGER; COUTINHO, LUIZ LEHMANN; PIRES DE CAMPOS ZUCCARI, DEBORA APARECIDA. RNA-Seq transcriptome analysis shows anti-tumor actions of melatonin in a breast cancer xenograft model. SCIENTIFIC REPORTS, v. 9, JAN 30 2019. Web of Science Citations: 0.