Production of cross-linked invertase aggregates from Saccharomyces cerevisiae as biocatalyst for the inversion of sucrose

• Grant number: 14/21510-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2015
• End date: December 31, 2015
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Paulo Waldir Tardioli
• Grantee: Maisa Bontorin Beltrame
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

In this research the enzyme invertase from Saccharomyces cerevisiae will be immobilized using the CLEA (cross-linked enzyme aggregate) technique. The main goal is to have an insoluble biocatalyst that is active, stable, and low cost, due to the fact that the immobilization technique not require the use of solid support, use unpurified enzymes and low cost reagents (precipitants and crosslinking agents). The immobilization parameters (precipitant reagent, concentration of the crosslinking agent, concentration of protein, and use of spacer protein) will be evaluated by fractional factorial experimental design (24-1). These parameters will be optimized by central composite rotational design (22), and the more active biocatalyst (invertase CLEA) will be characterized as pH and temperature of maximum catalytic activity, thermal stability, kinetic of sucrose hydrolysis, and operational stability in the batch production of inverted sugar syrup.