The white spot lesion is related to the mineral loss of enamel and is characterized by early manifestation of dental caries. The aim of this study is to assess white spot lesions after application of an infiltrating and submitted to challenges such as artificial brushing and pH cycling. Forty discs from bovine teeth will be divided into four groups with 10 specimens each: demineralized enamel, simulating artificial white spot lesion (negative control - GI), infiltrated demineralized enamel (GII); infiltrated demineralized enamel subjected to brushing (GIII); and infiltrated demineralized enamel subjected to pH cycling (GIV). Each specimen will have the own sound enamel as positive control. After the different challenges, the specimens will be evaluated for microhardness and roughness. The data will be submitted to ANOVA and Tukey's test for multiple comparisons (p d 0.05).
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