Real-time detection of nitric oxide in microdroplets via in situ SERS and SAXS spectroscopies

• Grant number: 15/01685-2
• Support type: Scholarships abroad - Research Internship - Post-doctor
• Effective date (Start): May 01, 2015
• Effective date (End): April 30, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal researcher: Marcelo Ganzarolli de Oliveira
• Grantee: Patricia Taladriz Blanco
• Supervisor abroad: Christopher Abell
• Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: University of Cambridge, England
• Associated to the scholarship: 13/07173-8 - Nanoparticles and hydrogels for the photochemical delivery of nitric oxide, BP.PD

Abstract

One of the main challenges in the synthesis of drug delivery carriers is the ability to precisely control the release of the drug. The release of Nitric Oxide (NO) from the decomposition of S-nitrosothiols (RSNOs) in the presence of gold nanoparticles is a promising approach since the amount of NO released is controlled by the surface of the nanoparticles (NPs). Combining the analytical technique Surface Enhanced Raman Spectroscopy (SERS), which allows the ultra detection of molecules, with microfluidics, offering a microenvironment to improve the control of the reactions, converts this interdisciplinary project a strong proposal for the real time detection of nitric oxide. In addition, in situ synchrotron radiation Small Angle X-Ray Scattering (SAXS) will be employed to study the evolution of the nanoparticle synthesis and further coating with the thiol in a single droplet.Therefore, this project involves two steps a) the synthesis of the most suitable gold nanoparticles in microdroplets and b) the reaction between the gold nanoparticles and RSNOs followed in situ by SERS. Further, both steps are intended to be tracked in situ by SAXS on the SAXS1 beam line at the LNLS. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ABALDE-CELA, SARA; TALADRIZ-BLANCO, PATRICIA; DE OLIVEIRA, MARCELO GANZAROLLI; ABELL, CHRIS. Droplet microfluidics for the highly controlled synthesis of branched gold nanoparticles. SCIENTIFIC REPORTS, v. 8, FEB 5 2018. Web of Science Citations: 18.