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Expression of opsins and melanopsins in snakes' retinas: molecular genetics and immunohistochemistry

Grant number: 14/25743-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2015
Effective date (End): October 31, 2019
Field of knowledge:Biological Sciences - Genetics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Dora Selma Fix Ventura
Grantee:Einat Hauzman
Home Institution: Instituto de Psicologia (IP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/26818-2 - Development and implementation of visual evaluation methods: clinical applications and animal models, AP.TEM
Associated scholarship(s):18/09321-8 - In vitro expression and spectral absorption measurements of the SWS1 photopigment of the Colubridae snake Helicops modestus, BE.EP.PD

Abstract

This project aims to answer important questions about the genetic and morphology of snakes retinas and continue the work of the PhD Einat Hauzman. Despite the great diversity of species of snakes, few studies have evaluated the specializations of their sensorial systems. During the student master's (Fapesp 2006/58514-6) were analyzed the density and distribution of photoreceptor and the ganglion cell layer cells (GCL) of two species of diurnal snakes. In the PhD project (Fapesp 2010/51670-8), the photoreceptors and GCL cells were quantified in retinas of 20 species of diurnal and nocturnal snakes, and the visual pigments genes were sequenced from the mRNA of retinas of 17 species. The different photoreceptor types were labeled by immunohistochemistry, using antibodies against opsins sensitive to short wavelengths, which labeled UV cones in snakes retinas (SWS1 opsin group), and against opsins sensitive to long wavelengths, which labeled L cones (LWS opsin group). The photopigment Rh1 expressed in snakes' retinas could not be labeled by any antibody. In this study, a specific antibody against the Rh1 photopigment will be produced and used to analyze the density and distribution of photoreceptors containing this visual pigment. Double or triple labeling with different anti-opsin antibodies will be carried out to analyze the opsins coexpression in a same photoreceptor and to quantify the different types of cones in a same retina. In this project, will also be analyzed the expression of the melanopsin genes, photopigment present in neurons of the inner retina, responsible for receiving luminous information from the environment and generate a variety of responses to light, that includes the regulation of circadian rhythms and the pupillary reflex. (AU)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GALANTE ROCHA DE VASCONCELOS, FELIPE TADEU; VILANI NAMAN, MARIA JULIA; HAUZMAN, EINAT; BARON, JEROME; VENTURA, DORA FIX; OLIVEIRA BONCI, DANIELA MARIA. LWS visual pigment in owls: Spectral tuning inferred by genetics. VISION RESEARCH, v. 165, p. 90-97, DEC 2019. Web of Science Citations: 0.
HAUZMAN, EINAT; KALAVA, VENKATASUSHMA; OLIVEIRA BONCI, DANIELA MARIA; VENTURA, DORA FIX. Characterization of the melanopsin gene (Opn4x) of diurnal and nocturnal snakes. BMC Evolutionary Biology, v. 19, n. 1 AUG 28 2019. Web of Science Citations: 0.
BITTENCOURT, GUIDO BARBIERI; HAUZMAN, EINAT; OLIVEIRA BONCI, DANIELA MARIA; VENTURA, DORA FIX. Photoreceptors morphology and genetics of the visual pigments of Bothrops jararaca and Crotalus durissus terrificus (Serpentes, Viperidae). VISION RESEARCH, v. 158, p. 72-77, MAY 2019. Web of Science Citations: 1.
SALGUEIRO BARBONI, MIRELLA TELLES; HAUZMAN, EINAT; NAGY, BALAZS VINCE; GOMES MARTINS, CRISTIANE MARIA; AHER, AVINASH J.; TSAI, I, TINA; OLIVERIA BONCI, DANIELA MARIA; VENTURA, DORA FIX; KREMERS, JAN. Electrodiagnosis of dichromacy. VISION RESEARCH, v. 158, p. 135-145, MAY 2019. Web of Science Citations: 0.
AHER, AVINASH J.; MARTINS, CRISTIANE M. G.; BARBONI, MIRELLA T. S.; NAGY, BALAZS V.; HAUZMAN, EINAT; BONCI, DANIELA M. O.; VENTURA, DORA F.; KREMERS, JAN. Electroretinographical determination of human color vision type. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, v. 35, n. 4, p. B92-B99, APR 1 2018. Web of Science Citations: 1.
HAUZMAN, E.; BONCI, D. M. O.; SUAREZ-VILLOTA, E. Y.; NEITZ, M.; VENTURA, D. F. Daily activity patterns influence retinal morphology, signatures of selection, and spectral tuning of opsin genes in colubrid snakes. BMC Evolutionary Biology, v. 17, DEC 11 2017. Web of Science Citations: 6.
SALGUEIRO BARBONI, MIRELLA TELLES; NAGY, BALAZS VINCE; GOMES MARTINS, CRISTIANE MARIA; OLIVERIA BONCI, DANIELA MARIA; HAUZMAN, EINAT; AHER, AVINASH; TSAI, TINA I.; KREMERS, JAN; VENTURA, DORA FIX. L-/M-cone opponency in visual evoked potentials of human cortex. JOURNAL OF VISION, v. 17, n. 9 AUG 2017. Web of Science Citations: 4.
GALANTE ROCHA DE VASCONCELOS, FELIPE TADEU; HAUZMAN, EINAT; HENRIQUES, LEONARDO DUTRA; KILPP GOULART, PAULO RONEY; GALVAO, OLAVO DE FARIA; SANO, RONALDO YUITI; SOUZA, GIVAGO DA SILVA; ALFARO, JESSICA LYNCH; DE LIMA SILVEIRA, LUIS CARLOS; VENTURA, DORA FIX; OLIVEIRA BONCI, DANIELA MARIA. A novel nonsense mutation in the tyrosinase gene is related to the albinism in a capuchin monkey (Sapajus apella). BMC GENETICS, v. 18, MAY 5 2017. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.