Acquired enamel film engineering for protection against tooth erosion: in vivo eva...
| Grant number: | 14/22430-0 |
| Support type: | Scholarships in Brazil - Doctorate |
| Effective date (Start): | March 01, 2015 |
| Effective date (End): | October 31, 2017 |
| Field of knowledge: | Health Sciences - Dentistry |
| Cooperation agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | Marília Afonso Rabelo Buzalaf |
| Grantee: | Luiza de Paula Silva Cassiano |
| Home Institution: | Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil |
Abstract This study, divided into 2 parts, was designed to answer the following questions: 1) does whole fluoridated milk protect more against enamel and dentine erosion than fat-free fluoridated milk? 2) does the protective effect of fluoridated milk against erosion follow a dose-response relationship? 3) is the treatment with whole or fat-free fluoridated milk before the first erosive challenge more protective against enamel and dentine erosion than the treatment after the first erosive challenge? 4) does the fat content of milk change the proteomic profile of the acquired pellicle, increasing its acid resistance? For Part I, bovine enamel and dentin specimens will be submitted to the following treatments (n=15/group): 0.9% NaCl solution (negative control)(T1); bovine whole milk with 0 ppm F (T2); bovine whole milk with 2.5 ppm F (T3); bovine whole milk with 5.0 ppm F(T4) ; bovine whole milk with 10.0 ppm F (T5); bovine fat-free milk with 0 ppm F(T6); bovine fat-free milk with 2.5 ppm F(T7); bovine fat-free milk with 5.0 ppm F(T8); bovine fat-free milk with 10.00 ppm F(T9); 0.05% NaF solution (positive control) (T10); bovine whole milk with 0 ppm F (milk before first erosive challenge) (T11); bovine whole milk with 2.5 ppm F (milk before first erosive challenge) (T12); bovine whole milk with 5.0 ppm F (milk before first erosive challenge) (T13); bovine whole milk with 10.0 ppm F (milk before first erosive challenge) (T14); bovine fat-free milk with 0 ppm F (milk before first erosive challenge) (T15); bovine fat-free milk with 2.5 ppm F (milk before first erosive challenge) (T16); bovine fat-free milk with 5.0 ppm F (milk before first erosive challenge) (T17); bovine fat-free milk with 10.0 ppm F (milk before first erosive challenge) (T18); 0.05% NaF solution (positive control; before first erosive challenge) (T19). Specimens will be submitted to demineralization-remineralization regimes, for 5 days, 4 times per day. For T1-T10, treatment will be performed after the first erosive challenge, while for T11-T19 it will be performed before the erosive challenge. The response variable will be wear, evaluated by profilometry (µm). For part II, 90 bovine enamel specimens and 90 bovine dentine specimens (8X8 mm) will be used; the specimens will be immersed in human saliva for acquired pellicle formation and divided into 3 groups (n = 30 per group): whole milk , fat-free milk or deionized water. The treatment will be: individual immersion of each specimen in 30 mL of whole milk, fat-free milk or deionized water for 1 min followed by immersion in 30 mL of slurry 1:1 of correspondent milk or deionized water and human saliva for 10 min. In sequence, they will be additionally incubated with human saliva for 50 min and washed. Of the 30 specimens/group, half will be used for erosive challenge and analysis of calcium in the solution and the remaining to proteomic analysis. The acquired pellicle will be formed on the specimens by exposure to pool of human stimulated saliva (from 3 donors). The pellicles will be collected with filter paper soaked in 3 % citric acid and processed for analysis by LC-ESI-MS/MS. (AU) | |