Structural and ultrastructural characterization of infection by Aeromonas hydrophila in Piaractus mesopotamicus

• Grant number: 15/01024-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2015
• End date: August 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Julieta Rodini Engracia de Moraes
• Grantee: Fausto de Almeida Marinho Neto
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Among the problems in fish farming stand out the bacterial diseases which are installed due to stress caused by environmental adverse conditions and handling which are responsible for slow growth and high mortality rates. Techniques such as light microscopy and Transmission Electron Microscopy (TEM) are used in the study of biological material, in particular, to examine structural changes, ultrastructural and cytopathic effects caused by bacteria and other microorganisms in several types of infections. This diagnostic procedure contributes to understand the host-pathogen interaction, which is very important to the understanding of the pathogenesis and development of strategies for intervention and disease control. This project aims to investigate sepsis through the study of infection, dissemination and colonization of A. hydrophila in blood and different organs of pacus (Piaractus mesopotamicus) and the serum lytic activity. For this, it will be used 170 pacus (weight 150.71 ± 56,73g and total length 18.27 ± 4.70 cm). Initially, we will determine the LD50 (LD50-96h) for A. hydrophila in pacus. Sepsis will be induced by administration of celomatic inoculum corresponding to the LD50 and a group injected by the same route with 0.65% saline sterile as a control. Before inoculation and 1, 3, 6 and 9 hours after inoculation, ten animals in each group (saline control and challenged) will be anesthetized for blood collection, necropsy and organ collection for light microscopy and transmission electron microscopy analysis, dissemination of the bacteria in the different compartments and to chart the growth curve of A. hydrophila. Throughout the experiment, the animals will be evaluated for clinical behavior changes. The results will be compared by analysis of variance (ANOVA) at 5% probability and the difference between means by Tukey test (p <0.05). (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARINHO NETO, Fausto de Almeida. Acute sepsis caused by Aeromonas hydrophila IN Piaractus mesopotamicus: morphological and microbiological characterization. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.