Incorporation of epigallocatechin gallate in etch-and-rinse adhesive system and its effect on flexural strength and bond durability to caries-affected dentin

• Grant number: 15/06259-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2015
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Flavia Lucisano Botelho Do Amaral
• Grantee: Renata Czech
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

The present in vitro study aims to evaluate the incorporation of epigallocatechin gallate (EGCG), a metalloproteinase-MMP inhibitor, in etch-and-rinse adhesive system and its effect on flexural resistance (FR) and bond durability to caries-affected dentin, by means of microtensile bond strength testing (MBS). For MBS, forty dentin slabs will be submitted to a microbiological caries model to induce caries formation. After infected caries removal, caries affected dentin surface will be acid conditioned (37% phosphoric acid) for 15 seconds and randomly divided into four groups according to the application mode of the MMP-inhibitory solution on dentin surface (n=10): EGCG-PRE - EGCG solution (200¼g/mL) applied before adhesive system (as a pre-treatment); EGCG-INC - EGCG solution incorporated to the etch-and-rinse adhesive system at a ratio of 200¼g/mL; CLX-PRE - 2% chlorhexidine digluconate solution applied before adhesive system (as a pre-treatment) (positive control); NT - Any treatment will be performed (negative control). Adhesive system will be applied according to manufacture's instruction and composite restorative will be placed on dentin surface. Dentin-resin blocks will be sectioned into 8 beam-shaped specimens (0.8mm2 area) that will be allocated into two groups, according to the moment of microtensile bond strength evaluation: 24 hours or after a 6-months period of water storage. For FR analysis, bar-shaped specimens (7mm x 2mm x 1mm - length x width x thickness) will be obtained with the two-step adhesive system, containing or not the EGCG solution (200¼g/mL). Both MBS and FR tests will be performed in a universal testing machine, operating at a 0.5 mm/min speed. Data will be statistically analyzed considering its distribution. If there is normality of errors distribution and homogeneity of variance, parametric tests will be applied; on the contrary, non-parametric testes will be adopted, both with 5% significance level.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CZECH, R.; OLIVEIRA, C. A. R.; FRANCA, F. M. G.; BASTING, R. T.; TURSSI, C. P.; AMARAL, F. L. B.. Incorporation of EGCG into an etch-and-rinse adhesive system: mechanical properties and bond strength to caries affected dentin. JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, v. 33, n. 22, JULY 2019. (15/06259-1)