Non-ulcerated or atypical cutaneous leishmaniasis caused by Leishmania infantum chagasi in Amapala municipality, Valle, Honduras: immune-histological characterization of the skin lesions

• Grant number: 15/01154-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2015
• End date: May 31, 2017
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcia Dalastra Laurenti
• Grantee: Carmen Maria Sandoval Pacheco
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the Americas, particularly in South America, infection with Leishmania (Leishmania) infantum chagasi cause subclinical manifestations and visceral leishmaniasis (VL), which if untreated is potentially fatal. In Honduras, the leishmaniasis by L. (L.) infantum chagasi causes visceral leishmaniasis (VL), but has also been reported the non-ulcerated or atypical cutaneous leishmaniasis (NUCL) caused by this specie of parasite. There are cases of non-ulcerated or atypical cutaneous leishmaniasis (LCA), also reported in other countries in Central America, such as Costa Rica, El Salvador and Nicaragua. In Honduras, there are endemic areas well identified of this non-ulcerated clinical variant, located in Choluteca and Valle departments, as well as the southern part of Francisco Morazán department, La Paz, El Paraiso and Olancho. We intend to do an active search of patients with non-ulcerated or atypical cutaneous leishmaniasis in the city of Amapala, Valle department, Honduras, from January to December 2015. After clinical examination, biopsies of skin lesions of these patients, which are defined as a papule, plaque or asymptomatic infiltrative nodule, with no ulceration, erythematous or skin color, with or without the presence of hypo-pigmented halo, will be collected, fixed in formalin solution 10% buffered 0.01M and processed by the usual techniques of microscopy optical. Histological sections stained by HE will be used to characterize the histopathological changes of the cutaneous lesions and another histological sections will be processed by immunohistochemistry using primary antibodies to dendritic cells (CD1a, CD11c), macrophages (CD68), T lymphocytes (CD4, CD8 and FoxP3) and intracellular markers (IL-10, TGF-b, IFN-g and iNOS) in order to evaluate the in situ immunity. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO FLORES, GABRIELA VENICIA; SANDOVAL PACHECO, CARMEN MARIA; TOMOKANE, THAISE YUMIE; OCHOA, WILFREDO SOSA; VALERIANO, CONCEPCION ZUNIGA; CASTRO GOMES, CLAUDIA MARIA; PEREIRA CORBETT, CARLOS EDUARDO; LAURENTI, MARCIA DALASTRA. Evaluation of Regulatory Immune Response in Skin Lesions of Patients Affected by Nonulcerated or Atypical Cutaneous Leishmaniasis in Honduras, Central America. Mediators of Inflammation, v. 2018, p. 7-pg., 2018-01-01. (14/50315-0, 15/01154-7)

ARAUJO FLORES, GABRIELA VENICIA; SANDOVAL PACHECO, CARMEN MARIA; TOMOKANE, THAISE YUMIE; OCHOA, WILFREDO SOSA; VALERIANO, CONCEPCION ZUNIGA; CASTRO GOMES, CLAUDIA MARIA; PEREIRA CORBETT, CARLOS EDUARDO; LAURENTI, MARCIA DALASTRA. Evaluation of Regulatory Immune Response in Skin Lesions of Patients Affected by Nonulcerated or Atypical Cutaneous Leishmaniasis in Honduras, Central America. Mediators of Inflammation, 2018. (15/01154-7, 14/50315-0)

SANDOVAL PACHECO, CARMEN M.; ARAUJO FLORES, V, GABRIELA; GONZALEZ, KADIR; DE CASTRO GOMES, CLAUDIA M.; PASSERO, LUIZ F. D.; TOMOKANE, THAISE Y.; SOSA-OCHOA, WILFREDO; ZUNIGA, CONCEPCION; CALZADA, JOSE; SALDANA, AZAEL; et al. Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2021, APR 12 2021. (14/50315-0, 17/24834-9, 15/01154-7, 18/04698-6)

SANDOVAL, CARMEN; ARAUJO, GABRIELA; SOSA, WILFREDO; AVALOS, SARA; SILVEIRA, FERNANDO; CORBETT, CARLOS; ZUNIGA, CONCEPCION; LAURENTI, MARCIA. In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, FEB 26 2021. (18/04698-6, 14/50315-0, 17/24834-9, 15/01154-7)

SANDOVAL PACHECO, CARMEN MARIA; ARAUJO FLORES, GABRIELA VENICIA; FAVERO FERREIRA, AUREA; SOSA OCHOA, WILFREDO; RIBEIRO DA MATTA, VANIA LUCIA; VALERIANO, CONCEPCION ZUNIGA; PEREIRA CORBETT, CARLOS EDUARDO; DALASTRA LAURENTI, MARCIA. Histopathological features of skin lesions in patients affected by non-ulcerated or atypical cutaneous leishmaniasis in Honduras, Central America. International Journal of Experimental Pathology, v. 99, n. 5, p. 249-257, OCT 2018. (15/01154-7, 14/50315-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PACHECO, Carmen Maria Sandoval. Non-ulcerated or atypical cutaneous leishmaniasis caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras: immunohistotopathological characterization of skin lesions. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.