Programmed cell death photoinduced mechanisms and different gene expression profiles

• Grant number: 15/07768-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2015
• End date: December 31, 2017
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Mauricio da Silva Baptista
• Grantee: Felipe Gustavo Ravagnani
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/50680-5 - Photosensitization in life sciences, AP.TEM

Abstract

Photodynamic therapy (PDT - Photodynamic therapy) is a treatment against several human diseases whose basic principle is the use of a photosensitive molecule, which is internalized by the cell and excited with visible light (600-800nm), generating several reactive species and inducing cell death in the target tissues. The main objective of the thematic research grant is to identify mechanisms to induce programed cell death, using light. In previous studies, we characterized ways to induce, with similar photosensitizers, distinct mechanisms of programmed cell death. This induction was shown to be dependent mainly on the cellular localization of the photosensitizer. Although the lab focus has always been in eukaryotic cells, we intend, in the coming years, to also apply these concepts in prokaryotes organisms. In this project we will compare qualita tively and quantitatively the gene expression induced by the photosensitivity reactions depending on the cellular localization of the photosensitizer and on the type of programmed cell death. Furthermore, we also aim to expand these studies to prokaryotic organisms.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALERIO, HELLEN PAULA; RAVAGNANI, FELIPE GUSTAVO; CANDELA, ANGELA PAOLA YAYA; DA COSTA, BRUNA DIAS CARVALHO; RONSEIN, GRAZIELLA ELIZA; DI MASCIO, PAOLO. Spatial proteomics reveals subcellular reorganization in human keratinocytes exposed to UVA light. ISCIENCE, v. 25, n. 4, p. 27-pg., 2022-04-15. (15/07768-7, 16/00696-3, 16/11430-4, 12/12663-1, 13/07937-8)

VALERIO, HELLEN PAULA; RAVAGNANI, FELIPE GUSTAVO; RONSEIN, GRAZIELLA ELIZA; DI MASCIO, PAOLO. A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes. SCIENTIFIC REPORTS, v. 11, n. 1, DEC 2 2021. (15/07768-7, 13/07937-8, 12/12663-1, 16/11430-4, 16/00696-3)