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Lipid microdomains in Langmuir-Blodgett films rich in alkaline phosphatase to obtain a Ti surface osteoinductive

Grant number: 15/00345-3
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Ana Paula Ramos
Grantee:Marco Aurélio Raz de Andrade
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Biomineralization is the term given to all the process of formation of minerals in living organisms. The union of one inorganic portion with an organic portion results composites with unique properties. The osseous tissue, for example, is made by one complexity of cells responsible for synthesis and maintenance of carbonated hydroxyapatite composite within collagen fibrils. The osseous tissue synthesis is regulated by a diversity of enzymes; being the alkaline phosphatase one of those enzymes with a key role in local supersaturation of phosphate ions on osseous matrix mineralization and regulates the inhibitors concentrations of this process. In this project, organic matrices made by phospholipids and addictives will be deposited upon metallic supports (titanium) by Langmuir-Blodgett technique. Those matrices will be used as a biomimetic system to insertion of alkaline phosphatase and analysis in its role on calcium phosphates growth, exposing those supports containing the matrices in solutions that contains phosphorylated substrates and mineralization nucleators. Then a series of analyses will be made, checking the morphologic characteristics of the films by microscopy (scanning electron, fluorescence and atomic force) and structural characteristics of the films by spectroscopy (infrared and x-ray dispersive energy), zeta potential and surface energy; assessing the immobilized enzyme activity and the cellular viability of osteoblastic cells on the Ti modified surface. With that we intend to obtain osteoinductive biomaterials with potential of clinical application, making possible the optimization of osseous prosthesis integration. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANDRADE, MARCO A. R.; DERRADI, RAFAEL; SIMAO, ANA M. S.; MILLAN, JOSE LUIS; RAMOS, ANA P.; CIANCAGLINI, PIETRO; BOLEAN, MAYTE. Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?. Archives of Biochemistry and Biophysics, v. 663, p. 192-198, MAR 15 2019. Web of Science Citations: 2.
FAVARIN, B. Z.; ANDRADE, M. A. R.; BOLEAN, M.; SIMAO, A. M. S.; RAMOS, A. P.; HOYLAERTS, M. F.; MILLAN, J. L.; CIANCAGLINI, P. Effect of the presence of cholesterol in the interfacial microenvironment on the modulation of the alkaline phosphatase activity during in vitro mineralization. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 155, p. 466-476, JUL 1 2017. Web of Science Citations: 9.
ANDRADE, MARCO A. R.; FAVARIN, BRUNO; DERRADI, RAFAEL; BOLEAN, MAYTE; SIMAO, ANA MARIA S.; MILLAN, JOSE LUIS; CIANCAGLINI, PIETRO; RAMOS, ANA P. Pendant-drop method coupled to ultraviolet-visible spectroscopy: A useful tool to investigate interfacial phenomena. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 504, p. 305-311, SEP 5 2016. Web of Science Citations: 3.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ANDRADE, Marco Aurélio Raz de. Lipid microdomains films rich in alkaline phosphatase in Langmuir-Blodgett to obtain a Ti surface osteoinductive.. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Ribeirão Preto.

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