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Evaluation of medial pterygoid muscle and brain structures related to orofacial nociception and emotional behavior in an animal model of chronic stress and masticatory hypofunction

Grant number: 15/03053-3
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): September 01, 2015
Effective date (End): November 02, 2018
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Elaine Aparecida Del Bel Belluz Guimarães
Grantee:Glauce Crivelaro Do Nascimento
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):18/05146-7 - Morphological differentiation of NG2 and astrocyte in striatum of rats under an experimental model of parkinsonism: efficacy of doxycycline on neuroinflammation process, BE.EP.PD


The patient presenting dental loss reveals an occlusal collapse influencing stomatognathic functions. Although stress is involved in the pathogenesis of chronic diseases and enable the masticatory muscle pain in patients, it is not well understood the mechanism by which muscle adapts to psychological and physiological stresses and which neural pathways are involved in orofacial muscle dysfunction due to stress and/or masticatory hypofunction. Among the orofacial musculoskeletal pain, it is possible to highlight those caused by temporomandibular disorders (TMD). This disorder has a multifactorial etiology, and malocclusion, trauma, inflammation and emotional stress are part of this range of causal factors. One of the most notable and recent areas that have been studied in relation to TMD is its involvement with emotional disturbances. This study aims to investigate the effect of variable chronic stress associated with unilateral extraction on the medial pterygoid muscle, brain and plasma corticosterone. The influence of pretreatment of animals with diazepam, a classical anxiolytic, will also be evaluated. Male Wistar rats will be used (200g) and they will be randomly divided into two groups: Malocclusion Groups (GM): rats exposed to left unilateral extraction and Group without Malocclusion (GS): rats without extraction. There will be the following subgroups (n = 8): no stress; chronic stress; no stress and treated with diazepam; chronic stress and treated with diazepam. The rats will be euthanized on the 24th day after the beginning of the experiment to obtain the medial pterygoid muscle to HE analysis, MET, MMPs, Laminin and ROS; specific brain regions for analysis of c-fos and fos-B and collection of blood for analysis of plasma corticosterone. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GARATTINI, EMANUELA G.; SANTOS, BRUNA M.; FERRARI, DANIELE P.; CAPEL, CAMILA P.; FRANCESCATO, HELOISA D. C.; COIMBRA, TEREZILA M.; LEITE-PANISSI, CHRISTIE R. A.; BRANCO, LUIZ G. S.; NASCIMENTO, GLAUCE C.. Propargylglycine decreases neuro-immune interaction inducing pain response in temporomandibular joint inflammation model. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 93, p. 90-101, . (15/03053-3, 17/03645-3, 16/09364-3, 16/17681-9)
RESTREPO FERNANDEZ, RODRIGO ALBERTO; LARA PEREIRA, YAMBA CARLA; IYOMASA, DANIELA MIZUSAKI; CALZZANI, RICARDO ALEXANDRE; ANDRADE LEITE-PANISSI, CHRISTIE RAMOS; IYOMASA, MAMIE MIZUSAKI; NASCIMENTO, GLAUCE CRIVELARO. Metabolic and vascular pattern in medial pterygoid muscle is altered by chronic stress in an animal model of hypodontia. Physiology & Behavior, v. 185, p. 70-78, . (12/22128-6, 15/03053-3)
LARA PEREIRA, YAMBA CARLA; NASCIMENTO, GLAUCE CRIVELARO; IYOMASA, DANIELA MIZUSAKI; RESTREPO FERNANDEZ, RODRIGO ALBERTO; CALZZANI, RICARDO ALEXANDRE; ANDRADE LEITE-PANISSI, CHRISTIE RAMOS; NOVAES, PEDRO DUARTE; IYOMASA, MAMIE MIZUSAKI. Exodontia-induced muscular hypofunction by itself or associated to chronic stress impairs masseter muscle morphology and its mitochondrial function. MICROSCOPY RESEARCH AND TECHNIQUE, v. 82, n. 5, p. 530-537, . (15/03053-3, 12/22128-6)
NASCIMENTO, GLAUCE C.; MALZONE, BRUNO L.; IYOMASA, DANIELA M.; PEREIRA, YAMBA C. L.; ISSA, JOAO PAULO M.; LEITE-PANISSI, CHRISTIE R. A.; WATANABE, II-SEI; IYOMASA, MAMIE M.; FUENTES, RAMON; DEL BEL, ELAINE; et al. Beneficial effects of benzodiazepine on masticatory muscle dysfunction induced by chronic stress and occlusal instability in an experimental animal study. SCIENTIFIC REPORTS, v. 10, n. 1, . (12/22128-6, 15/03053-3)
NASCIMENTO, GLAUCE CRIVELARO; DE PAULA, BRUNA BALBINO; FERRARI, DANIELE P.; IYOMASA, DANIELA MIZUSAKI; PEREIRA, YAMBA C. L.; PEDRAZZI, JOAO F.; BORTOLANZA, MARIZA; ISSY, ANA CAROLINA; MARDEGAN ISSA, JOAO PAULO; LEITE-PANISSI, CHRISTIE R. A.; et al. Upregulation of FosB/Delta FosB in limbic circuits after tooth exodontia-induced occlusal instability in an experimental model of unpredictable chronic stress. Brain Research Bulletin, v. 176, p. 142-150, . (17/24304-0, 18/05146-7, 14/25029-4, 15/03053-3)
NASCIMENTO, GLAUCE CRIVELARO; LUCAS, GUILHERME; ANDRADE LEITE-PANISSI, CHRISTIE RAMOS. Emerging role of microglia and astrocyte in the affective-motivational response induced by a rat model of persistent orofacial pain. Brain Research Bulletin, v. 195, p. 13-pg., . (15/03053-3, 11/07718-9)

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