Characterization in vitro and functional of autologous platelet-rich plasma (PRP) as a therapeutic product in different processing conditions

• Grant number: 15/05544-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2015
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Joyce Maria Annichino-Bizzacchi
• Grantee: Letícia Queiroz da Silva Justo
• Host Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Platelet-rich plasma (PRP) it is a level of plasma that has a platelet concentration above baseline platelet number of a healthy individual. The PRP has lately been used as autologous therapeutic product. The main component that seems to be associated with their therapeutic effect is the presence of growth factors. Although, diverse protocols are performed, in order to amplify the therapeutic effect proposed by PRP. The high variability in the methodology induces heterogeneous results that affect the data from available research to compare evaluation of effectiveness of the product. A better understanding of PRP functions according to its components can provide the election of a PRP, with some minimum requirements, which would make it effective in clinical applications in which it is used. The objective of the study is to compare in vitro two processes for obtaining PRP that has different technical characteristics, both are going to be compared for their ability to platelet concentration, potential release of growth factors, through activation by thrombin and functional ability to promote proliferation and cell signaling. Twenty healthy male and female individuals will be included on this study, with a age from 20 to 55 years. The protocols will be processes in parallel, the first one will be selected as the most widely used for studies and the second was recently described and appear to concentrate higher levels of growth factors. The data obtained from this study will lead to selection of a better protocol for future therapeutic applications of PRP.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, LETICIA QUEIROZ; DE LIMA MONTALVAO, SILMARA APARECIDA; JUSTO-JUNIOR, AMAURI DA SILVA; ROSENBERIS CUNHA JUNIOR, JOSE LUIZ; HUBER, STEPHANY CARES; OLIVEIRA, CAROLINA CALIARI; ANNICHINO-BIZZACCHI, JOYCE MARIA. Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh platelet-rich plasma. REGENERATIVE MEDICINE, v. 13, n. 7, p. 775-784, OCT 2018. (15/05544-4)