|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||October 01, 2015|
|Effective date (End):||February 28, 2017|
|Field of knowledge:||Health Sciences - Pharmacy - Pharmaceutical Technology|
|Principal Investigator:||Carolina Patrícia Aires|
|Grantee:||Erika Reiko Hashimoto Kawakita|
|Home Institution:||Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil|
Biofilms cause several diseases, as caries and periodontitits.Oral biofilm is naturally formed and removed by brushing but if that sanitation is inadequate, buccal products with antimicrobials are recommended. Antimicrobial effect of chitosan is well known, but its actions on biofilms are not. Thus, the purpose of the present study is to avaluate antimicrobial activity of gels and microparticles of chitosan in planctonic cells and biofims of Streptococcus mutans UA 159. Primarily, both gels and microparticles of chitosan will be obtained and characterized by rheological analysis, zeta potential and texture profile. Then, obtained material will be evaluated regarding minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for planctonic cells. For biofilm studies, they will be formed for 5 days in glass slides, mimicking physiological conditions of feast and famine episodes. At day 3 of experiment, biofilms will be exposed to followings treatments (n=3): a) NaCl 0.9% as negative control; b) chlorhexidine digluconate solution 0,12% as positive control; c) chitosan gel at different concentrations to be defined from MIC; d) chitosan microparticles at different concentrations to be defined from MIC. Biofilm acidogenicity will be monitored daily. In the end of experimental period, bacterial viability will be evaluated and polysaccharides will be quantified. For between-groups comparison, initially homogeneity and results variability will be analyzed. Data will be statistically analyzed and the level of significance settled will be 5%.