Deciphering the common bean resistance against the anthracnose disease: identification of kinase client-relationships and characterization of signaling network in an incompatible interaction P. vulgaris/C. lindemuthianum

• Grant number: 15/23533-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 01, 2016
• End date: January 31, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Tsai Siu Mui
• Grantee: Danielle Gregorio Gomes Caldas
• Supervisor: Steven P. Briggs
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Institution abroad: University of California, San Diego (UC San Diego), United States
• Associated to the scholarship: 13/06301-2 - Deciphering the common bean resistance against the anthracnose disease: Transcriptome and identification of genes responsive to the compatible and incompatible interaction Phaseolus vulgaris/Colletotricuhm lindemuthianum, BP.PD

Abstract

The ability of plants to defend themselves against pathogens is of prime importance. In the case of an aggressive pathogen attack, a fast response could mean the difference between survival and death, so molecular events linked to an early response are critical for the survival of the plant. Phosphorylation of existing proteins is a method whereby a cell can respond quickly to a stimulus. In our previous studies, we found a much faster expression of some resistance-genes in an incompatible interaction between common bean and Colletotrichum lindemuthianum than in a compatible interaction. In addition, deep transcriptomic analysis revealed a series of Protein Kinases being up-regulated in this pathosystem. In order to study this interaction in a molecular signaling level, we propose to study the phosphoproteome of the same samples already studied in the transcriptomic level and identify Kinase-clients relationships for the innate immune response of common bean to C. lindemuthianum invasion. For this, total protein will be extracted and prepared for phosphoproteomic analysis, MS analyzed and the phosphosites identified. In parallel, Protein Kinases will be selected from previous work, cloned and purified, and used in a KiC assay (Kinase Client Assay). As result, we hope to advance in our principal aim on deciphering the common bean resistance against the anthracnose disease, going to an upper level of gene expression, identifying phosphosites in its defense signaling and mainly, discovering the kinase-client proteins relationships in this process. (AU)