Photothermal heterodyne image Microscopy single molecule tracking of antimicrobial peptide

• Grant number: 15/22901-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: January 06, 2016
• End date: June 05, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Edgar Julian Paredes-Gamero
• Grantee: Marcus Vinicius Buri
• Supervisor: David Garth Fernig
• Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Institution abroad: University of Liverpool, England
• Associated to the scholarship: 12/20989-4 - Molecular mechanisms studies in differentiation and cell death by antimicrobial peptides using leukemic and adenocarcinoma cells, BP.DR

Abstract

The antimicrobial peptides (AMPs) are defence molecules found in different organisms. Besides its antimicrobial activities, it has been shown that they possess potential antitumor activity. Our group described the ß-hairpin AMPs (protegrin, tachyplesin, polyphemusin and gomesin) cytotoxicity as well as some mechanisms triggered by them related to cell death of tumor cell lines such as K562, B16 and CHO (Chinese hamster ovary). We described that these peptides can cause direct membrane disruption in high concentrations while, in lower concentration, triggering different types of cell death after entry into tumor cells. Currently, we are extending the studies on ß-hairpin AMPs (protegrin, tachyplesin, polyphemusin and gomesin) and their anti-tumor activity concerning membrane interaction and organelle targeting by PHI (phototermal heterodyne image) microscopy and morphological, molecular and biochemical events of cell death. PHI microscopy is a technique that permits to follow a single molecule tracking such as AMPs. Thus, the use of PHI microscopy will allow us to monitor the entry of AMPs across cell membrane and follow these AMPs inside of cell determinate organelle targeting. Additionally, in the end of the project we also aim to analyze all data with computational mathematic multiparametric analysis, such as Bayesian nets, in order to evaluate all the pathways in an integrated way.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BURI, MARCUS VINICIUS; SPERANDIO, LETICIA PAULINO; DE SOUZA, KAMYLLA F. S.; ANTUNES, FERNANDA; REZENDE, MARINA MASTELARO; MELO, CARINA MUCCIOLO; PINHAL, MARIA A. S.; BARROS, CARLOS C.; FERNIG, DAVID G.; YATES, EDWIN A.; et al. Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by beta-Hairpin Antimicrobial Peptides. ACS APPLIED BIO MATERIALS, v. 4, n. 8, p. 6488-6501, AUG 16 2021. (13/09068-7, 15/22901-5, 12/20989-4)

BURI, MARCUS V.; DIAS, CAROL C.; BARBOSA, CHRISTIANO M. V.; NOGUEIRA-PEDRO, AMANDA; RIBEIRO-FILHO, ANTONIO C.; MIRANDA, ANTONIO; PAREDES-GAMERO, EDGAR J.. Gomesin acts in the immune system and promotes myeloid differentiation and monocyte/macrophage activation in mouse. Peptides, v. 85, p. 41-45, NOV 2016. (13/09068-7, 15/22901-5, 11/17584-0, 12/20989-4)