Structural and biophysical characterization of animal toxins

• Grant number: 15/24010-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2015
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Maria Cristina Nonato
• Grantee: Joane Kathelen Rustiguel Bonalumi
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis, AP.TEM

Abstract

Structural biology, a research area focusing the structural characterization of biological macromolecules, has a crucial role in the functional characterization of proteins and nucleic acids. A combination of recombinant DNA, biochemical and biophysical techniques, including specific tools for the determination of 3-dimensional structures provide the basis for the development of studies of the correlation between structure and function. The present project aims the investigation of structure-function relationship of different animal toxins provided and under investigation by our scientific partners, Professor Eliane Candiani Arantes Braga and Professor Suely Vilela, including phospholipases, L-aminoacid oxidases and hialuronidases from different sources. The isolated proteins will be submitted to structural studies by a combination of X-ray diffraction techniques and biophysical techniques such as circular dichroism, thermoflour, and electron paramagnetic resonance (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FELICIANO, PATRICIA R.; RUSTIGUEL, JOANE K.; SOARES, RICARDO O. S.; SAMPAIO, SUELY V.; CRISTINA NONATO, M.. Crystal structure and molecular dynamics studies of L-amino acid oxidase from Bothrops atrox. Toxicon, v. 128, p. 50-59, MAR 15 2017. (15/24010-0, 09/10454-3, 13/14988-8)

WIEZEL, GISELE A.; RUSTIGUEL, JOANE K.; MORGENSTERN, DAVID; ZOCCAL, KARINA E.; FACCIOLI, LUCIA H.; CRISTINA NONATO, M.; UEBERHEIDE, BEATRIX; ARANTES, ELIANE C.. Insights into the structure, function and stability of bordonein-L, the first L-amino acid oxidase from Crotalus durissus terrificus snake venom. Biochimie, v. 163, p. 33-49, AUG 2019. (14/03332-7, 11/23236-4, 15/24010-0, 14/23285-3, 14/06170-8, 14/07125-6)