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Multi-omics analyzes of bacterial contaminations in first-generation ethanolic fermentations

Grant number: 15/13431-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2016
Effective date (End): February 28, 2019
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal researcher:Carlos Alberto Labate
Grantee:Lucas Souza Lopes
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


The Ethanol has a great strategic importance for the brazilian energy sector due to its renewable nature and great capacity to generate employment and income. Furthermore, the use of ethanol as a fuel is very interesting, since this decreases the brazilian dependence on fossil fuels. Currently, one of the main problems faced in ethanol production is the presence of contaminating microorganisms in the industrial environment. Their presence in the fermentation can cause a reduction in efficiency in the ethanol production, competition for essential nutrients and increase of toxic compounds levels that cause yeast flocculation. Even with these problems, most of the ethanol production process in Brazil are based on a complex interaction between the yeast S. cerevisiae and contaminating organisms. In this sense, this project aims to carry out DNA sequencing, assembly of genomes and complete annotation of two isolates of bacterial contaminants (Lactobacillus sp. And Acetobacter sp.), Identified directly in brazilian ethanol production Industry. Furthermore, it is also aims to peform the sequencing of CAT-1 transcripts S. cerevisiae and these contaminants when coexisting conditions, in fermentation trials conducted in semi-industrial reactors. Finally, from these studies are expected to elucidate the interactions and dynamics of these microrganisms, and in a subsequent step, to develop new methods for the control of contaminants in the industry which are more specific and efficient than those currently used.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LOPES, Lucas Souza. Multi-omics analyzes of bacterial contaminations in first-generation ethanolic fermentations. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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