Optical Coherence Tomography signal autocorrelation analysis algorithm applied to blood flow monitoring in mice with Metabolic Syndrome

• Grant number: 15/15775-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2016
• End date: October 02, 2019
• Field of knowledge: Engineering - Biomedical Engineering
• Principal Investigator: Anderson Zanardi de Freitas
• Grantee: Lucas Ramos de Pretto
• Host Institution: Instituto de Pesquisas Energéticas e Nucleares (IPEN). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 13/26113-6 - Micromachining with ultrashort laser pulses applied to the production and control of optofluidic circuits, AP.TEM
• Associated scholarship(s): 16/17342-0 - Application of speckle analysis techniques in OCT for optical diagnosis in vivo, BE.EP.DR

Abstract

The Optical Coherence Tomography (OCT) is an interferometry-based diagnostic imaging technique that makes use of non-ionizing electromagnetic radiation. It is characterized as non-invasive, non-contact, high-resolution (few microns) and with good penetration depth (a few millimeters in biological tissues). For these characteristics, it stands out as an attractive technique for biological applications because it does not present risks to patients and does not require prior sample preparation. Furthermore, the OCT technique has a wide variety of analysis aimed to obtain data on underlying flow in samples (such as microcirculation in biological tissues). One of these techniques is based on speckle, type of noise that occurs naturally in the OCT signal. The analysis of speckle intensity fluctuation through time provides data concerning the motion of scatterers and, therefore, concerning sampled flows. One way to analyze the speckle is through its temporal autocorrelation, an approach that will be studied and improved in this project. It will, also, be applied to the monitoring of microcirculation in animal models of metabolic syndrome (MS), a condition that groups several risk factors for cardiovascular disease, diabetes mellitus and hyperglycemia. High blood glucose levels may trigger abnormal responses in the microcirculatory system, which can be observed through speckle analysis in OCT, making the technique an interesting diagnostic tool for MS. In addition, in a collaboration with Prof. James G. Fujimoto from MIT - Massachusetts Institute of Technology. Another technique based on speckle, the OCT Angiography (OCTA) will be studied during the collaboration for 12 months.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PRETTO, LUCAS R.; MOULT, ERIC M.; ALIBHAI, A. YASIN; CARRASCO-ZEVALLOS, OSCAR M.; CHEN, SIYU; LEE, BYUNGKUN; WITKIN, ANDRE J.; BAUMAL, CAROLINE R.; REICHEL, ELIAS; DE FREITAS, ANDERSON ZANARDI; et al. Controlling for Artifacts in Widefield Optical Coherence Tomography Angiography Measurements of Non-Perfusion Area. SCIENTIFIC REPORTS, v. 9, JUN 24 2019. (16/17342-0, 15/15775-3)

RAELE, MARCUS PAULO; DE PRETTO, LUCAS RAMOS; DE ROSSI, WAGNER; VIEIRA, JR., NILSON DIAS; SAMAD, RICARDO ELGUL. Focus Tracking System for Femtosecond Laser Machining using Low Coherence Interferometry. SCIENTIFIC REPORTS, v. 9, MAR 12 2019. (15/24878-0, 15/15775-3)

ALIBHAI, A. YASIN; DE PRETTO, LUCAS R.; MOULT, ERIC M.; OR, CHRIS; ARYA, MALVIKA; MCGOWAN, MITCHELL; CARRASCO-ZEVALLOS, OSCAR; LEE, BYUNGKUN; CHEN, SIYU; BAUMAL, CAROLINE R.; et al. QUANTIFICATION OF RETINAL CAPILLARY NONPERFUSION IN DIABETICS USING WIDE-FIELD OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY. RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES, v. 40, n. 3, p. 412-420, MAR 2020. (16/17342-0, 15/15775-3)

RAELE, MARCUS PAULO; DE PRETTO, LUCAS RAMOS; ZEZELL, DENISE MARIA. Soldering mask laser removal from printed circuit boards aiming copper recycling. WASTE MANAGEMENT, v. 68, p. 475-481, OCT 2017. (15/15775-3, 15/24878-0)

DE PRETTO, LUCAS RAMOS; AMARAL, MARCELLO MAGRI; DE FREITAS, ANDERSON ZANARDI; RAELE, MARCUS PAULO. Nondestructive evaluation of fused filament fabrication 3D printed structures using optical coherence tomography. RAPID PROTOTYPING JOURNAL, v. 26, n. 10, p. 1853-1860, NOV 3 2020. (17/21851-0, 15/15775-3, 19/19465-0, 18/20226-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PRETTO, Lucas Ramos de. Development of quantitative diagnostic techniques based on decorrelation of speckle present in Optical Coherence Tomography signals. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Pesquisas Energéticas e Nucleares (IPEN/BT) São Paulo.