Effect of low level laser therapy (LLLT) in an experimental model of pulmonary allergic inflammation induced house dust mite (HDM): study of dosimetry

• Grant number: 15/23152-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2016
• End date: June 30, 2018
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Ana Paula Ligeiro de Oliveira
• Grantee: Nicole Cristine Rigonato de Oliveira
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 12/16498-5 - Effect of low level laser therapy in experimental models of pulmonary chronic diseases, AP.JP

Abstract

Currently asthma has a high prevalence and a high economic and social cost. In this context, several experimental models have been proposed, aiming to advance knowledge about the pathophysiological processes of asthma, as well as the discovery of new therapeutic options. Therapy with Low Power Laser (LBP) is a relatively new effective therapy , with very low cost and no side effects. In this project we aim to study some parameters in animals with asthma undergoing therapy with LBP. To achieve that, BALB/c male mice were divided into 6 experimental groups: (control, n = 20; LBP, n = 20; HDM, n = 20; and HDM + LLL (1J, 3J, 5J and 7,5J; n = 20/group). The protocol used for the induction of asthma will be administered HDM intranasally (on days 0, 7, 14, 21, 28, 35 and 42). Laser therapy is applied before challenge with HDM. Twenty-four hours after the last HDM challenge and laser irradiation, animals will be anesthetized, tracheotomized, cannulated and bronchoalveolar lavage collected for analysis of total and differential counting of cells and the release of cytokines (IL-4, IL-5, IL-10 e IL-13). We will also evaluate the participation of the transcription factors STAT6 and GATA3 (intact and phosphorylated), and the evaluation of T cells and eosinophils by flow citometry and lung mechanics will be performed.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; et al. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2018. (12/16498-5, 15/13486-4, 12/15165-2, 14/14604-8, 15/23152-6)

CANDEO, LARISSA CARBONERA; RIGONATO-OLIVEIRA, NICOLE CRISTINE; BRITO, AURILEIA APARECIDA; MARCOS, RODRIGO LABAT; FRANCA, CRISTIANE MIRANDA; SANTOS FERNANDES, KRISTIANNE PORTA; MESQUITA-FERRARI, RAQUEL AGNELLI; BUSSADORI, SANDRA KALIL; VIEIRA, RODOLFO PAULA; LINO-DOS-SANTOS-FRANCO, ADRIANA; et al. Effects of periodontitis on the development of asthma: The role of photodynamic therapy. PLoS One, v. 12, n. 11, NOV 16 2017. (15/20535-1, 12/16498-5, 15/23152-6, 15/00830-9)

RIGONATO-OLIVEIRA, NICOLE CRISTINE; DE BRITO, AURILEIA APARECIDA; VITORETTI, LUANA BEATRIZ; MORAES, GABRIEL DE CUNHA; GONCALVES, TAWANY; HERCULANO, KARINE ZANELLA; ALVES, CINTIA ESTEFANO; LINO-DOS-SANTOS-FRANCO, ADRIANA; AIMBIRE, FLAVIO; VIEIRA, RODOLFO PAULA; et al. Effect of Low-Level Laser Therapy (LLLT) in Pulmonary Inflammation in Asthma Induced by House Dust Mite (HDM): Dosimetry Study. INTERNATIONAL JOURNAL OF INFLAMMATION, 2019. (15/23152-6)

MORAES, GABRIEL DA CUNHA; VITORETTI, LUANA BEATRIZ; DE BRITO, AURILEIA APARECIDA; ALVES, CINTIA ESTEFANO; RIGONATO DE OLIVEIRA, NICOLE CRISTINE; DIAS, ALANA DOS SANTOS; TELES MATOS, YVES SILVA; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; FRANCO OLIVEIRA, LUIS VICENTE; DA PALMA, RENATA KELLY; et al. Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2018, p. 8-pg., 2018-01-01. (12/16498-5, 14/14604-8, 15/23152-6, 15/13486-4, 12/15165-2)

RIGONATO-OLIVEIRA, NICOLE CRISTINE; DE BRITO, AURILEIA APARECIDA; VITORETTI, LUANA BEATRIZ; MORAES, GABRIEL DE CUNHA; GONCALVES, TAWANY; HERCULANO, KARINE ZANELLA; ALVES, CINTIA ESTEFANO; LINO-DOS-SANTOS-FRANCO, ADRIANA; AIMBIRE, FLAVIO; VIEIRA, RODOLFO PAULA; et al. Effect of Low-Level Laser Therapy (LLLT) in Pulmonary Inflammation in Asthma Induced by House Dust Mite (HDM): Dosimetry Study. INTERNATIONAL JOURNAL OF INFLAMMATION, v. 2019, p. 12-pg., 2019-01-01. (15/23152-6)