Purification and identification of peptides with biological activities, released from the hydrolysis of casein goats for protease synthesized by filamentous fungi

• Grant number: 16/10289-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2016
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Roberto da Silva
• Grantee: José Erick Galindo Gomes
• Supervisor: Maria Manuela Estevez Pintado
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Institution abroad: Universidade Católica Portuguesa, Porto (UCP), Portugal
• Associated to the scholarship: 14/13700-3 - Evaluation of biological activity of peptides released from the hydrolysis of caprine casein by proteases synthesized by filamentous fungi, BP.DR

Abstract

ABSTRACTIn recent years, consumer demand for foods known as functional, i.e., that promote health benefits beyond basic nutritional care, is growing constantly. It is considered as a potential strategy for prevention of chronic diseases, for their physiological benefits or reduce the risk of these diseases, regardless of their nutritional properties. In this sense, bioactive peptides have a high potential for use in the food industry, especially for this purpose. Consequently, there have encouragement from the industry and academia to develop techniques and processes with different proteolytic sources for the release of these substances. Hydrolysis of casein by specific microbial proteases such as filamentous fungi, results in the release of various peptides with different biological activities. Thus, the screening and evaluation of these peptides for their application may represent an important contribution to the functional foods sector. It is of fundamental importance for a research project that involves this type of biomolecule, identify their amino acid sequences to understand what types of interaction peptide / target may occur to achieve this purpose. For the realization of a work of this magnitude, it is essential that the purification experiments, determination of biological activity and identification of peptides with such activities are carried out within the same workplace, ensuring the stability of molecules and thus preserving its bioactive potential. Thus, this proposal Exchange Research Internship Abroad (BEPE) is working together with Professor Dra. Maria Manuela Estevez painted from School of Biotechnology, located at the Portuguese Catholic University (UCP) in Porto. - Portugal. The researcher plays an important role in research on bioactive compounds, including peptides biologically active and extensive experience in purification and identification of these molecules. In addition, the Food Bioprocessing Laboratory of the Center for Biotechnology and Fine Chemicals from UCP, has all scientific apparatus necessary for this work. Therefore, our objective is to hydrolyze the goat casein by proteases from Mucor subitilissimus URM 4133 and Mucor guilliermondii URM 5848 fungi, purify the peptides released from this hydrolysis and identify their amino acid sequences, with the purpose to evaluate which the interactions between amino acids are responsible for certain biological activities. The study will assess the potential of these peptides to perform antimicrobial activity, antioxidant and anti-hypertensive. Thus, separation and purification techniques such as electrophoresis, high-performance liquid chromatography (RP-HPLC) using iodine array detectors, mass spectrometers of the kind LC-MS / MS and mass spectrometers the type MALDI-TOF / TOF , they will be used. Subsequently, the peptides are sequenced compared with the peptide already registered in databases such as the NCBI BLAST protein, UniProt (Universal Protein Resource) and BioPep to verify whether they have been described or are novel sequences. Thus contributing both to the records in the literature of these biomolecules, as in order to improve the achievement of bioactive peptides. (AU)