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Influence of nicotine associated with obesity in the alveolar bone repair of rats: histomorphometric analysis

Grant number: 15/26633-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2016
Effective date (End): June 30, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Cristiane Furuse
Grantee:Karoliny Canhet Paterno
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

White adipose tissue, initially recognized as an organ of passive energy accumulation, has important endocrine function that influence bone metabolism. Animal and clinical studies have shown a negative influence of smoking on development, maintenance and repair of bone tissue. Little is known, however, about the Influence of obesity associated with chronic use of nicotine in the bone repair process. This project aims to analyze the alveolar repair process in obese rats associated with the chronic use of nicotine. For this, 10 adult (50-60 days) of approximately 200g male rats (Wistar) will be used. First, obesity will be developed with high-fat diet M42% Kcal pelleted with casein (Rhoster) supplemented with cafeteria diet for 3 months. The rats will be divided into two groups, control group of obese rats without chronic use of nicotine (Sigma) (n = 5), and experimental group, obese rats with chronic use (n = 5). In the experimental group, 5mg nicotine/kg will be subcutaneously administered daily in the last 30 days of fattening stage to euthanasia. The animals will have extracted the upper right incisor and will be euthanized on the 28th day after the surgery. Histomorphometric analysis will be performed in digital photomicrographs of the medim third of the dental alveolus in HE staining section with the help of the program Image J. The data will be statistically analyzed by ANOVA (analysis of variance), as the level of significance p <0, 05.