Histological analysis of the cicatrization in chemical ulcers after treatment the copaiba oil (Copaifera Hayne multijuga), with concentration of the 3%

• Grant number: 15/23154-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Adriana Torrecilhas Jorge
• Grantee: Thays Thaciane Souza Pinto
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated research grant: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species, AP.TEM

Abstract

Brazil is nursery of medicinal plants; however, the efficacy of the majority of these plants has yet to be scientifically proven. Several studies on copaiba oil have analyzed its anti-inflammatory, analgesic and healing effects, but no reports are available in the literature regarding its use and efficacy in ophthalmology. The aim of this study is to analyze the healing effects of copaiba oil (Copaifera multijuga Hayne) on corneal chemical ulcers. Chemicals ulcers will be induced with sodium hydroxide solution (NAOH) 1N. NAOH will be placed in the central position of the cornea for 80 seconds. It will be used 24 Wistar rats, subdivided into 4 groups (G3, GT, GE, GL), with 6 animals in each one. In group three (G3) will be used copaiba oil 3%; Tween group (GT) will be used Tween 80 at 5% solution; Epitezan group (GE), will use solution, ophthalmic ointment (Epitezan®) and Lubricant group (GL), ocular lubricant. The administration of medications will be established at intervals of 6 hours for 72 hours. After the treatments, the animals will be submitted to euthanasia with pentobarbital sodium and the ocular bulbs will be collected by subconjunctival enucleation surgery. Samples will be analyzed in light microscopy.