Use of membrane models to evaluate the biodegradability of chitosan

• Grant number: 16/09720-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2016
• End date: April 30, 2019
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Anderson Fiamingo
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

Chitosan is a biocompatible, biodegradable polysaccharide widely applied in several types of industry, especially biological applications. In the latter, its activity depends on the interaction with cell membranes, whose mechanisms at the molecular level have not been completely elucidated yet. Chitosan action depends on multiple parameters, such as average degree of acetylation, acetylation pattern, molecular weight and derivatization. In this project, chitosan and its derivatives will be produced with well-defined parameters for a detailed investigation of its effects on membrane models represented by Langmuir and Langmuir-Blodgett (LB) films of phospholipids. Lysozyme and other enzymes will be employed to evaluate the interactions between chitosan and membranes in order to probe degradation. The results obtained with the films will be correlated with the known biological activity of chitosan. Another important aim is to identify molecular-level processes responsible for side effects and/or secondary effects on the body, which is relevant to society owing to the indiscriminate use of chitosan as a supplement for weight loss and other purposes. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, ANDRESSA R.; FIAMINGO, ANDERSON; PEDRO, RAFAEL DE O.; CAMPANA-FILHO, SERGIO P.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N.. Enhanced chitosan effects on cell membrane models made with lipid raft monolayers. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 193, SEP 2020. (17/03503-4, 13/14262-7, 18/00878-0, 16/09720-4)

FACCHINATTO, WILLIAM MARCONDES; DOS SANTOS, DANILO MARTINS; FIAMINGO, ANDERSON; BERNARDES-FILHO, RUBENS; CAMPANA-FILHO, SERGIO PAULO; DE AZEVEDO, EDUARDO RIBEIRO; COLNAGO, LUIZ ALBERTO. Evaluation of chitosan crystallinity: A high-resolution solid-state NMR spectroscopy approach. Carbohydrate Polymers, v. 250, DEC 15 2020. (17/24465-3, 16/20970-2, 17/20973-4, 16/09720-4, 19/13656-8)

FACCHINATTO, WILLIAM MARCONDES; GARCIA, RODRIGO HENRIQUE DOS SANTOS; SANTOS, DANILO MARTINS DOS; FIAMINGO, ANDERSON; FLORES, DOUGLAS WILLIAM MENEZES; CAMPANA-FILHO, SERGIO PAULO; AZEVEDO, EDUARDO RIBEIRO DE; COLNAGO, LUIZ ALBERTO. Fast-forward approach of time-domain NMR relaxometry for solid-state chemistry of chitosan. Carbohydrate Polymers, v. 256, MAR 15 2021. (16/09720-4, 17/20973-4, 19/13656-8)

FACCHINATTO, WILLIAM MARCONDES; FIAMINGO, ANDERSON; DOS SANTOS, DANILO MARTINS; CAMPANA-FILHO, SERGIO PAULO. Characterization and physical-chemistry of methoxypoly (ethylene glycol)-g-chitosan. International Journal of Biological Macromolecules, v. 124, p. 828-837, MAR 1 2019. (16/20970-2, 17/20973-4, 10/02526-1, 16/09720-4)