Exercise training effects on SHR treated with dexamethasone: role of miRNAs

• Grant number: 16/12532-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2016
• End date: August 31, 2020
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Sandra Lia do Amaral Cardoso
• Grantee: Naiara Araújo Herrera
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• Associated scholarship(s): 18/06998-7 - EXERCISE TRAINING PREVENTS RAREFACTION INDUCED BY DEXAMETHASONE: ROLE OF MITOCHONDRIA, BE.EP.DR

Abstract

Hypertension (H) is a multifactorial disease characterized by high and sustained levels of blood pressure (BP), classified as primary and secondary H. Dexamethasone (DEX) is a synthetic glucocorticoid with potent anti-allergic and anti-inflammatory effects and H is one of its unwanted side-effect. Spontaneously hypertensive rats (SHR) treated with DEX may have higher mortality risk due to H exacerbation. Microcirculation rarefaction is present in primary H (SHR) and in DEX induced H and is associated with angiogenic and apoptotic pathways alterations. More recently, miRNAs has being considered important in primary H genesis; however, its role in SHR treated with DEX has not yet being investigated. On the other hand, physical training (T) has been recommended crucial strategy to control hypertension. The aim of this study is to investigate if T is able to attenuate BP exacerbation in SHR treated with DEX and if this effect is associated with microcirculation miRNAs expression. Normotensive (Wistar) and hypertensive rats (SHR) will be submitted to eight weeks of T on a treadmill (60% of maximum physical capacity, 5 days a week, one hour per day) or kept sedentary. During 14 days, after eight weeks of T, animals will be treated with DEX (50µg/kg per day, s.c.) or saline. Body weight will be measured weekly during the T and daily during DEX treatment. Resting BP and arterial stiffness will be measured after DEX treatment. Subsequently, tibialis anterior muscle and left ventricle will be removed and processed for capillary density and capillary-to-fiber ratio, as well as, for VEGF, PI3K, eNOS, Bcl-2 and caspase-3-cleaved proteins production and miRNAs-16, -21, -126, -155, -205, -221 and -222 gene expression evaluations. Results will be presented as mean ± mean standard error. ANOVA two way and Tukey post-hoc will be performed for comparison among groups (p <0.05).

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TARDELLI, LIDIELI P.; DUCHATSCH, FRANCINE; HERRERA, NAIARA A.; VICENTINI, CARLOS ALBERTO; OKOSHI, KATASHI; AMARAL, SANDRA L.. Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats. JOURNAL OF APPLIED TOXICOLOGY, v. 41, n. 10, p. 1673-1686, OCT 2021. (18/00567-4, 16/12532-5, 17/00509-1, 17/14405-3)

HERRERA, NAIARA A.; DUCHATSCH, FRANCINE; TARDELLI, LIDIELI P.; DIONISIO, THIAGO J.; SANTOS, CARLOS F.; AMARAL, SANDRA L.. Dexamethasone Does Not Inhibit Treadmill Training-Induced Angiogenesis in Myocardium: Role of MicroRNA-126 Pathway. Journal of Cardiovascular Pharmacology, v. 76, n. 6, p. 708-714, DEC 2020. (18/06998-7, 17/00509-1, 16/12532-5, 17/14405-3, 15/03965-2)

HERRERA, NAIARA ARAUJO; DUCHATSCH, FRANCINE; KAHLKE, ALLISON; AMARAL, SANDRA LIA; VASQUEZ-VIVAR, JEANNETTE. In vivo vascular rarefaction and hypertension induced by dexamethasone are related to phosphatase PTP1B activation not endothelial metabolic changes. Free Radical Biology and Medicine, v. 152, p. 689-696, MAY 20 2020. (18/06998-7, 16/12532-5, 17/00509-1, 17/14405-3)

TARDELLI, LIDIELI P.; DUCHATSCH, FRANCINE; HERRERA, NAIARA A.; VICENTINI, CARLOS ALBERTO; OKOSHI, KATASHI; AMARAL, SANDRA L.. Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats. JOURNAL OF APPLIED TOXICOLOGY, FEB 2021. (18/00567-4, 16/12532-5, 17/14405-3, 17/00509-1)

TARDELLI, LIDIELI P. P.; DUCHATSCH, FRANCINE; HERRERA, NAIARA A. A.; RUIZ, THALLES FERNANDO R.; PAGAN, LUANA U. U.; VICENTINI, CARLOS A. A.; OKOSHI, KATASHI; AMARAL, SANDRA L. L.. Benefits of combined exercise training on arterial stiffness and blood pressure in spontaneously hypertensive rats treated or not with dexamethasone. FRONTIERS IN PHYSIOLOGY, v. 13, p. 15-pg., 2022-08-15. (17/14405-3, 17/00509-1, 16/12532-5, 18/00567-4)

HERRERA, NAIARA A.; DUCHATSCH, FRANCINE; TARDELLI, LIDIELI P.; DIONISIO, THIAGO J.; SHINOHARA, ANDRE L.; SANTOS, CARLOS F.; AMARAL, SANDRA LIA. MicroRNA-126 upregulation, induced by training, plays a role in controlling microcirculation in dexamethasone treated rats. Molecular and Cellular Endocrinology, v. 505, APR 5 2020. (18/06998-7, 17/14405-3, 16/12532-5, 17/00509-1, 15/03965-2)