Implementation of a tetrad dissection method for Saccharomyces cerevisiae based on flow cytometry

• Grant number: 16/12852-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2016
• End date: November 30, 2018
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Gonçalo Amarante Guimarães Pereira
• Grantee: Monique Furlan
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 17/24957-3 - Development of a fluorescence-based approach for the high-throughput isolation of Kluyveromyces marxianus recombinant haploids, BE.EP.IC

Abstract

Yeasts adapted to industrial conditions are considered appropriate platforms for the development of commercial strains applied to the production of chemical derivatives of renewable raw materials, in particular biofuels. However, understanding genetic mechanisms related to robustness or combine industrially relevant features for crossings require the collection and analysis of a large number of segregating. In Saccharomyces cerevisiae, obtaining haploid typically occurs by manual dissection of tetrads, an extremely laborious procedure depending on the number of haploid that is required. In this context, the implementation of techniques forhigh-throughput tetrad dissection has great impact on reducing the time necessary for the study or development of new strains. Therefore, it is intended to implement a method of analysis and dissecting tetrads based on flow cytometry and apply it to the study of genes relating to thermotolerance observed in industrial strains.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORADINI, V, ALESSANDRO L.; BEZERRA DE MELLO, FELLIPE DA SILVEIRA; FURLAN, MONIQUE; MANEIRA, CARLA; CARAZZOLLE, MARCELO F.; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae. BIOTECHNOLOGY FOR BIOFUELS, v. 14, n. 1, DEC 16 2021. (16/12852-0, 16/02506-7, 15/06677-8, 14/26719-4, 18/03403-2, 13/08293-7)

BEZERRA DE MELLO, FELLIPE DA SILVEIRA; VENEGA CORADINI, ALESSANDRO LUIS; CARAZZOLLE, MARCELO FALSARELLA; MANEIRA, CARLA; FURLAN, MONIQUE; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. Genetic mapping of a bioethanol yeast strain reveals new targets for hydroxymethylfurfural- and thermotolerance. MICROBIOLOGICAL RESEARCH, v. 263, p. 13-pg., 2022-08-02. (16/12852-0, 15/06677-8, 13/08293-7, 16/02506-7, 14/26719-4, 18/03403-2)