Application the Bacillus subtilis subpsp. Spizizenii ATCC 6633 in production of y-PGA

• Grant number: 16/06587-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2016
• End date: September 30, 2017
• Field of knowledge: Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
• Principal Investigator: Valquiria de Campos
• Grantee: Guilherme Zeponi
• Host Institution: Instituto de Ciência e Tecnologia. Universidade Estadual Paulista (UNESP). Campus de Sorocaba. Sorocaba , SP, Brazil

Abstract

The biodegradable materials arise as an alternative to reduce the generation of waste, as in the treatment of public water supply, where aluminum sulfate generates high volume of sludge. This work aims to evaluate the production of acid ³-polyglumatic from the fermentation of soybean. The experiments will consist in assessing the efficiency of Bacillus subtilis in producing ³-polyglumatic acid, using conventional farming (BRS 284), the transgenic cultivar (BRS 359RR) and the cultivar of second generation (BRS 1001IPRO). It is expected that the soybean cultivars possess differences in centesimal composition, which will influence the production of ³-PGA. After establishing the cultivar which best meets the needs of micro-organisms, fermentation will take place the optimization of the production process of the biopolymer, varying different factors such as time, temperature, concentration of p.10536-10540, agitation and initial pH. This experimental line will contribute to implementation of the aid for Regular Search FAPESP n° 2015/02650-8.