Molecular and crystalline structures and their effect on the enzymatic susceptibility of different granular starches

• Grant number: 16/22136-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: March 01, 2017
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Celia Maria Landi Franco
• Grantee: Marcia Maria de Souza Moretti
• Supervisor: Robert Goulston Gilbert
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Institution abroad: University of Queensland, Brisbane (UQ), Australia
• Associated to the scholarship: 14/20521-8 - Extraction, characterization and hydrolysis of polysaccharides of Landoltia punctata biomass to obtain fermentable sugars, BP.PD

Abstract

Starch is one of the main macromolecular constituents of plants. It is a cheap, renewable and biodegradable raw material used in the food industry, in many non-food applications and as a source of energy after conversion to bioethanol. In an effort to explore novel starch sources for fuel ethanol production, Landoltia punctata a duckweed strain is being studied under different growing conditions for starch accumulation. In the last decade, studies have been performed using duckweed starch for ethanol production by the conventional route (gelatinization/liquefaction and saccharification of starch). However, few studies on duckweed starch molecular structure and none work on its conversion to reducing sugars by a granular starch hydrolyzing enzyme have been performed. Thus, this project proposes to study the enzymatic susceptibility of granular starch and molecular and crystalline organization (including lamellar structure) of the native e hydrolyzed starches from duckweed, corn, cassava, potato, and sweet potato using size-exclusion chromatography (SEC), fluorophore-assisted carbohydrate electrophoresis (FACE), small-angle X-ray scattering (SAXS), and 13C cross-polarization magic angle spinning magnetic resonance spectroscopy (13C CP/MAS NMR).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA MORETTI, MARCIA MARIA; YU, WENWEN; ZOU, WEI; LANDI FRANCO, CELIA MARIA; ALBERTIN, LILIANE LAZZARI; SCHENK, PEER M.; GILBERT, ROBERT G.. Relationship between the molecular structure of duckweed starch and its in vitro enzymatic degradation kinetics. International Journal of Biological Macromolecules, v. 139, p. 244-251, OCT 15 2019. (16/22136-0)