Effect of laser intensity low in growth factor expression in fibroblasts of the pulp deciduous teeth

• Grant number: 16/20662-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2017
• End date: December 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Letícia Kagiyama Moraes
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of this study is to investigate the Low Intensity Laser effects of different energy densities on viability in fibroblasts of the pulp deciduous teeth; mRNA expression of VEGF-A. Human deciduous teeth samples of pulp tissue will be obtained and grown in specific culture. After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: Group I (1,2 J/cm2 - 05 mW - 10s), II (2,5 J/cm2 - 05 mW - 20s), III (3,7 J/cm2 - 05 mW - 30s), IV (5,0 J/cm2 - 05 mW - 40s), V (6,2 J/cm2 - 05 mW - 50s), C+ e C-. The techniques used for the analysis will be MTT, Crystal Violet and RT-PCR. The values in triplicate will be analyzed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test when obey normal and non-normal distribution, respectively. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)