Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods

• Grant number: 15/13527-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2017
• End date: November 04, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Nilton Erbet Lincopan Huenuman
• Grantee: Miriam Rodriguez Fernandes
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 17/16754-5 - Characterization of Plasmids Associated with the Spread of the Colistin Resistance mcr-1 gene in Clinically Significant Enterobacteriaceae from South American and European Countries, BE.EP.DR

Abstract

Escherichia coli strains producing ²-lactamase extended spectrum (ES²L) of CTX-M type are endemic in Brazil, prevalent in cases of nosocomial infection and outpatient. Recently, strains producing CTX-M have spread to urban environments and pets and / or production, additionally affecting agribusiness. The versatility and variety of pathogenic or contaminant species as is inherent in its position as commensal microbiota of a wide variety of organisms. Thus, the selection and dissemination of ES²L genotypes within the species, is a predictable genetic event that has been facilitated by the acquisition of blaCTX-M genes, mobilized by plasmids. This study aims to elucidate aspects still unknown on the ancestral relationship and pan-resistoma plasmids that carry genes of the CTX-M type (blaCTX-M-2, blaCTX-M-8 and M-blaCTX-15) strains Escherichia coli isolated from clinical specimens (human and veterinary), the microbiota of humans and companion animals, production animals and urban aquatic environments in Brazil. In addition, plasmid curing methods will be evaluated in vitro and in vivo (invertebrate model Galleria mellonela) in order to propose solutions applicable in clinical practice and agribusiness, to reduce the problem of Plasmid-mediated resistance associated with high rates of morbidity and mortality.

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, MIRIAM R.; SELLERA, FABIO P.; CUNHA, MARCOS P. V.; LOPES, RALF; CERDEIRA, LOUISE; LINCOPAN, NILTON. Emergence of CTX-M-27-producing Escherichia coli of ST131 and clade C1-M27 in an impacted ecosystem with international maritime traffic in South America. Journal of Antimicrobial Chemotherapy, v. 75, n. 6, p. 1647-1649, JUN 2020. (15/13527-2, 16/08593-9, 14/11523-7)

GOLDBERG, DAPHNE W.; FERNANDES, MIRIAM R.; SELLERA, FABIO P.; COSTA, DANIELA G. C.; LOUREIRO BRACARENSE, ANA PAULA; LINCOPAN, NILTON. Genetic background of CTX-M-15-producing Enterobacter hormaechei ST114 and Citrobacter freundii ST265 co-infecting a free-living green turtle (Chelonia mydas). ZOONOSES AND PUBLIC HEALTH, v. 66, n. 5, p. 540-545, AUG 2019. (15/13527-2, 16/08593-9)

FERNANDES, MIRIAM R.; SELLERA, FABIO P.; MOURA, QUEZIA; ESPOSITO, FERNANDA; SABINO, CAETANO P.; LINCOPAN, NILTON. Identification and genomic features of halotolerant extended-spectrum-beta-lactamase (CTX-M)-producing Escherichia coli in urban-impacted coastal waters, Southeast Brazil. Marine Pollution Bulletin, v. 150, JAN 2020. (15/13527-2, 16/08593-9, 16/03044-7)

FERNANDES, MIRIAM R.; CERDEIRA, LOUISE; SILVA, MEIRE M.; SELLERA, FABIO P.; MUNOZ, MARIA; JUNIOR, FELICIO G.; AZEVEDO, SERGIO S.; POWER, PABLO; GUTKIND, GABRIEL; LINCOPAN, NILTON. Novel mcr-5.3 variant in a CTX-M-8-producing Escherichia coli ST711 isolated from an infected horse. Journal of Antimicrobial Chemotherapy, v. 73, n. 12, p. 3520-3522, DEC 2018. (16/08593-9, 15/21325-0, 15/13527-2)

FERNANDES, MIRIAM R.; SELLERA, FABIO P.; MOURA, QUEZIA; GASPAR, VITOR C.; CERDEIRA, LOUISE; LINCOPAN, NILTON. International high-risk clonal lineages of CTX-M-producing Escherichia coli F-ST648 in free-roaming cats, South America. INFECTION GENETICS AND EVOLUTION, v. 66, p. 48-51, DEC 2018. (15/13527-2)

SELLERA, ABIO P.; FERNANDES, MIRIAM R.; RUIZ, REGINA; FALLEIROS, ANA C. M.; RODRIGUES, FERNANDA P.; CERDEIRA, LOUISE; LINCOPAN, NILTON. Identification of KPC-2-producing Escherichia coli in a companion animal: a new challenge for veterinary clinicians. Journal of Antimicrobial Chemotherapy, v. 73, n. 8, p. 2259-2261, AUG 2018. (16/08593-9, 15/21325-0, 15/13527-2)

SELLERA, FABIO P.; FERNANDES, MIRIAM R.; MOURA, QUEZIA; SOUZA, TIAGO A.; NASCIMENTO, CRISTIANE L.; CERDEIRA, LOUISE; LINCOPAN, NILTON. Draft genome sequence of an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST644 isolated from a footpad infection in a Magellanic penguin (Spheniscus magellanicus). JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 12, p. 88-89, MAR 2018. (16/08593-9, 15/13527-2)

SILVA, MEIRE M.; SELLERA, FABIO P.; FERNANDES, MIRIAM R.; MOURA, QUEZIA; GARINO, FELICIO; AZEVEDO, SERGIO S.; LINCOPAN, NILTON. Genomic features of a highly virulent, ceftiofur-resistant, CTX-M-8-producing Escherichia coli ST224 causing fatal infection in a domestic cat. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 15, p. 2-pg., 2018-12-01. (16/08593-9, 15/13527-2)

SARTORI, LUCIANA; SELLERA, FABIO P.; MOURA, QUEZIA; CARDOSO, BRENDA; CERDEIRA, LOUISE; LINCOPAN, NILTON. Multidrug-resistant CTX-M-15-positive Klebsiella pneumoniae ST307 causing urinary tract infection in a dog in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 19, p. 2-pg., 2019-12-01. (16/08593-9, 15/13527-2)

DE CARVALHO, MARCELO P. N.; FERNANDES, MIRIAM R.; SELLERA, FABIO P.; LOPES, RALF; MONTE, DANIEL F.; HIPPOLITO, ALICIA G.; MILANELO, LILIANE; RASO, TANIA F.; LINCOPAN, NILTON. International clones of extended-spectrum beta-lactamase (CTX-M)-producing Escherichia coli in peri-urban wild animals, Brazil. TRANSBOUNDARY AND EMERGING DISEASES, v. 67, n. 5, p. 1804-1815, SEP 2020. (15/13527-2, 16/03044-7, 16/08593-9)

DOS ANJOS, CAROLINA; SABINO, CAETANO P.; BUERIS, VANESSA; FERNANDES, MIRIAM R.; POGLIANI, FABIO C.; LINCOPAN, NILTON; SELLERA, FABIO P.. Antimicrobial blue light inactivation of international clones of multidrug-resistant Escherichia coli ST10, ST131 and ST648. Photodiagnosis and Photodynamic Therapy, v. 27, p. 51-53, SEP 2019. (15/13527-2, 16/08593-9, 16/25095-2)

ESPOSITO, FERNANDA; FERNANDES, MIRIAM R.; LOPES, RALF; MUNOZ, MARIA; SABINO, CAETANO P.; CUNHA, MARCOS P.; SILVA, KETRIN C.; CAYO, RODRIGO; MARTINS, WILLAMES M. B. S.; MORENO, ANDREA M.; et al. Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential. Journal of Clinical Microbiology, v. 55, n. 12, p. 3454-3465, DEC 2017. (16/08593-9, 15/13527-2)

SELLERA, FABIO P.; FERNANDES, MIRIAM R.; SABINO, CAETANO P.; DE FREITAS, LAURA M.; DA SILVA, LUCIANO C. B. A.; POGLIANI, FABIO C.; RIBEIRO, MARTHA S.; HAMBLIN, MICHAEL R.; LINCOPAN, NILTON. Effective treatment and decolonization of a dog infected with carbapenemase (VIM-2)-producing Pseudomonas aeruginosa using probiotic and photodynamic therapies. VETERINARY DERMATOLOGY, v. 30, n. 2, p. 170+, APR 2019. (14/24581-5, 16/08593-9, 15/13527-2)

SELLERA, FABIO P.; FERNANDES, MIRIAM R.; FUGA, BRUNA; FONTANA, HERRISON; VASQUEZ-PONCE, FELIPE; GOLDBERG, DAPHNE W.; MONTE, DANIEL F.; RODRIGUES, LARISSA; CARDENAS-ARIAS, ADRIANA R.; LOPES, RALF; et al. Phylogeographical Landscape of Citrobacter portucalensis Carrying Clinically Relevant Resistomes. MICROBIOLOGY SPECTRUM, v. 10, n. 2, p. 7-pg., 2022-04-01. (19/15578-4, 20/08224-9, 15/13527-2)

DE CARVALHO, MARCELO P. N.; MOURA, QUEZIA; FERNANDES, MIRIAM R.; SELLERA, FABIO P.; PAGOTTO, ANA HELENA; STUGINSKI, DANIEL R.; CASTRO, RAFAEL A.; SANT'ANNA, SAVIO S.; GREGO, KATHLEEN F.; LINCOPAN, NILTON. Genomic features of a multidrug-resistant Enterobacter cloacae ST279 producing CTX-M-15 and AAC(6 ')-Ib-cr isolated from fatal infectious stomatitis in a crossed pit viper (Bothrops alternatus). JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 15, p. 2-pg., 2018-12-01. (16/08593-9, 15/13527-2)

SELLERA, FABIO P.; FERNANDES, MIRIAM R.; MOURA, QUEZIA; LOPES, RALF B.; SOUZA, TIAGO A.; CERDEIRA, LOUISE; LINCOPAN, NILTON. Draft genome sequence of a bla(CMY-2)/IncI1-harbouring Escherichia coli D:ST457 isolated from coastal benthic organisms. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 14, p. 2-pg., 2018-09-01. (16/08593-9, 15/13527-2)