Pasteurization influence on the bacterium's ability to express genes of diarrheal toxins production in milk experimentally contaminated with Bacillus cereus

• Grant number: 16/23141-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2017
• End date: March 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
• Principal Investigator: Ana Maria Centola Vidal
• Grantee: Alenia Naliato Vasconcellos
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The Bacillus Cereus is a bacterium that has enzymatic action and not only can lead to technological problems in the milk production chain but also is a threat to public health once it produces toxins that can cause a diarrheal syndrome. Such bacteria have the characteristic of being resistant to many thermal treatments used in the processing of milk and derivatives in addition to synthesize biofilms and adhere to equipment and utensils. Despite the genes related with toxins produced by microorganism are already mentioned in literature, there are no studies that evaluate how the expression of certain genes can be affected by the pasteurization of the contaminated milk by B. Cerus. Therefore, the objective of this project is to determine if the expression of genes related to diarrheal toxins production can be affected by the pasteurization of a previously contaminated milk.